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Journal of Clinical Microbiology, January 2002, p. 5-9, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.5-9.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay

Michaela A. Riddell,1,2* Jennie A. Leydon,1 Mike G. Catton,1 and Heath A. Kelly1

Victorian Infectious Diseases Reference Laboratory/WHO Western Pacific Measles Regional Reference Laboratory, North Melbourne, Australia,1 Department of Paediatrics, University of Melbourne, Parkville 3052, Victoria, Australia2

Received 26 June 2001/ Returned for modification 16 August 2001/ Accepted 9 October 2001

The optical densities (ODs) of 216 dried venous blood (DVB) samples submitted to the Victorian Infectious Diseases Reference Laboratory as part of enhanced measles surveillance were compared to the ODs of the corresponding serum samples collected at the same time. DVB samples, stored for up to 24 months at 4°C, were tested by the Dade Behring Enzygnost Anti-Measles-Virus/IgM immunoassay. Elution and testing conditions were optimized with the use of spiked DVB samples. The assay showed an overall sensitivity of 90.2% and a specificity of 98.8% for DVB samples compared to the results for serum. When the results were analyzed according to the length of time that the DVB sample had been stored, the assay was 100% sensitive and 97% specific according to the ODs for those samples stored for less than 6 months compared to the results for the corresponding serum samples, with 97.7% agreement between the results for the two sample types. These results demonstrate the potential for the use of DVB samples for the diagnosis of measles in routine diagnostic laboratories.

* Corresponding author. Mailing address: Epidemiology and Public Health, Victorian Infectious Diseases Reference Laboratory, 10 Wreckyn St., North Melbourne 3051, Victoria, Australia. Phone: 61 3 9342 2686. Fax: 61 3 9342 2665. E-mail: michaela.riddell{at}mh.org.au.

Journal of Clinical Microbiology, January 2002, p. 5-9, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.5-9.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2002 by the American Society for Microbiology. All rights reserved.