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Journal of Clinical Microbiology, October 2002, p. 3635-3640, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3635-3640.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Herpes Simplex Virus Type 2-Specific Immunoglobulin G Antibodies in African Sera by Using Recombinant gG2, Western Blotting, and gG2 Inhibition

Wayne Hogrefe,1* Xin Su,1 James Song,1 Rhoda Ashley,2 and Lilly Kong1

Focus Technologies, Cypress, California,1 Department of Laboratory Medicine, University of Washington, Seattle, Washington2

Received 13 May 2002/ Returned for modification 15 June 2002/ Accepted 21 July 2002

Sera (n = 781) from four African countries were used to determine the prevalence of herpes simplex virus type 2 (HSV-2) antibodies by using the HerpeSelect HSV-2 enzyme-linked immunosorbent assay (ELISA; Focus Technologies) and Western blotting (WB). Also, an HSV inhibition assay was developed to evaluate the discordant sample results between HerpesSelect and WB. The seroprevalence of HSV-2 ranged from 17% in the South African panel to nearly 70% in panels from Kenya, Uganda, and Zimbabwe. Overall, HerpeSelect was 100% sensitive and 88% specific compared to WB and 100% sensitive and 96% specific compared to the inhibition assay. There was 100% concordance among all three assays for samples from South Africa and Zimbabwe. The discordant results occurred in samples from Kenya and Uganda.

* Corresponding author. Mailing address: Focus Technologies, 5785 Corporate Ave., Cypress, CA 90630. Phone: (714) 220-1900. Fax: (714) 821-3364. E-mail: Whogrefe{at}focusanswers.com.

Journal of Clinical Microbiology, October 2002, p. 3635-3640, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3635-3640.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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