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Journal of Clinical Microbiology, October 2002, p. 3689-3693, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3689-3693.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of a Cytomegalovirus Glycoprotein B Recombinant Enzyme Immunoassay To Discriminate between a Recent and a Past Infection

M. J. Sipewa, P. Goubau, and M. Bodéus*

Department of Microbiology, Unit of Virology, Université Catholique de Louvain, UCL-ESP 3055, 1200 Brussels, Belgium

Received 30 March 2001/ Returned for modification 28 May 2002/ Accepted 8 July 2002

Fetal damage following cytomegalovirus (CMV) intrauterine infection is mostly linked to primary infection. To differentiate primary infection from nonprimary infection, immunoglobulin M (IgM) tests are not reliable enough, and measurement of the IgG avidity appears to be the method that is the most widely used at present. In the present study the performance of the Vidas (bioMérieux) avidity assay was compared with that of a new enzyme immunoassay based on the use of a recombinant CMV glycoprotein B protein (Biotest).

* Corresponding author. Mailing address: Department of Microbiology, Unit of Virology, Université Catholique de Louvain, Clos Chapelle aux Champs 30, UCL-ESP 3055, 1200 Brussels, Belgium. Phone: 32-2.764.34.20. Fax: 32-2.764.31.63. E-mail: Bodeus{at}mblg.ucl.ac.be.

Journal of Clinical Microbiology, October 2002, p. 3689-3693, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3689-3693.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2002 by the American Society for Microbiology. All rights reserved.