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Journal of Clinical Microbiology, October 2002, p. 3712-3719, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3712-3719.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

IS1311 and IS1245 Restriction Fragment Length Polymorphism Analyses, Serotypes, and Drug Susceptibilities of Mycobacterium avium Complex Isolates Obtained from a Human Immunodeficiency Virus-Negative Patient

Lenka Dvorska,1 Milan Bartos,1 Oldrich Ostadal,2 Jarmila Kaustova,3 Ludmila Matlova,1 and Ivo Pavlik1*

Veterinary Research Institute, Brno,1 Department of Clinical Immunology and Allergology, Faculty Hospital, Olomouc,2 Department of Diagnostic of Mycobacteria, Regional Institute of Hygiene, Ostrava, Czech Republic3

Received 30 January 2002/ Returned for modification 20 March 2002/ Accepted 8 July 2002

Six isolates of Mycobacterium avium of genotype dnaJ+ IS901- IS1311+ IS1245+ and serotypes 6 (n = 1), 6/9, (n = 2), and 9 (n = 3) were obtained within a 5-month period from a human immunodeficiency virus-negative patient treated for tuberculosis. The isolates were identified with PvuII restriction fragment length polymorphism (RFLP) analysis as a single IS1311 RFLP type and six different IS1245 RFLP types. Six separate colonies/clones obtained by subculture from each of the six isolates were tested for MICs of a set of 10 drugs. This report documents the appearance of isolates that are resistant to antimycobacterial drugs as the duration of therapy increases. Because isolates recovered from the patient following longer duration of treatment were more likely to be resistant to more antimycobacterial drugs, we would conclude that there was selection for antimycobacterial drug-resistant isolates. Analyses of all 36 clones identified three IS1311 and 22 IS1245 types forming three clusters. Tests of 105 environmental samples collected in the home and the work place of the patient yielded 16 mycobacterial isolates, of which one M. avium from soil was of genotype dnaJ+ IS901+ IS1311+ IS1245+ and serotype 2, and the second M. avium from a vacuum cleaner was of genotype dnaJ+ IS901- IS1311+ IS1245+ and serotype 9. Overall analyses of the results did not reveal any relation between serotype, RFLP type, and drug susceptibility. Based on the course of the disease in the patient and different serotypes, IS1311 and IS1245 RFLP types of isolates of M. avium we suppose represent polyclonal infection.

* Corresponding author. Mailing address: Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic. Phone: 420 5 4132 1241. Fax: 420 5 4121 1229. E-mail: pavlik{at}vri.cz.

Journal of Clinical Microbiology, October 2002, p. 3712-3719, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3712-3719.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Domingos, M., Amado, A., Botelho, A. (2009). IS1245 RFLP analysis of strains of Mycobacterium avium subspecies hominissuis isolated from pigs with tuberculosis lymphadenitis in Portugal. Vet Rec. 164: 116-120 [Abstract] [Full Text]  
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  • Turenne, C. Y., Wallace, R. Jr., Behr, M. A. (2007). Mycobacterium avium in the Postgenomic Era. Clin. Microbiol. Rev. 20: 205-229 [Abstract] [Full Text]  
  • Matlova, L., Dvorska, L., Ayele, W. Y., Bartos, M., Amemori, T., Pavlik, I. (2005). Distribution of Mycobacterium avium Complex Isolates in Tissue Samples of Pigs Fed Peat Naturally Contaminated with Mycobacteria as a Supplement. J. Clin. Microbiol. 43: 1261-1268 [Abstract] [Full Text]  


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