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Journal of Clinical Microbiology, October 2002, p. 3750-3752, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3750-3752.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ability of the MicroScan Rapid Gram-Negative ID Type 3 Panel To Identify Nonenteric Glucose-Fermenting and Nonfermenting Gram-Negative Bacilli

Caroline M. O'Hara* and J. Michael Miller

Epidemiology and Laboratory Branch, Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 29 May 2002/ Returned for modification 18 June 2002/ Accepted 24 July 2002

The MicroScan Rapid Neg ID3 panel is designed for the identification of Enterobacteriaceae and nonenteric glucose-fermenting and nonfermenting gram-negative bacilli. We evaluated this panel for its ability to identify gram-negative non-Enterobacteriaceae bacteria. A total of 134 strains, representing 26 genera and 42 species, were taken from storage at -70oC, passaged three times before testing, and inoculated into the panels according to the manufacturer's directions before being inserted into a Walk/Away 96 instrument loaded with version 22.28 software. At the end of the initial 2.5-h incubation period, 89 isolates (66.4%) were correctly identified at a probability level of >=85%. After additional testing recommended by the manufacturer was completed, another 11 isolates (8.2%) were correctly identified at probability levels of >=85%. Twenty-five (18.7%) isolates were correctly identified after additional testing, but the probability levels were less than 85%. Two isolates were unidentified, and seven (5.2%) were incorrectly identified. The seven misidentified strains were not concentrated in any one genus. With an accuracy approaching 75%, this product may be used for the identification of the commonly isolated non-Enterobacteriaceae bacteria but may present problems in identification of other non-glucose-fermenting gram-negative bacilli.

* Corresponding author. Mailing address: Mailstop C16, Atlanta, GA 30333. Phone: (404) 639-2316. Fax: (404) 639-3822. E-mail: cmo1{at}cdc.gov.

Journal of Clinical Microbiology, October 2002, p. 3750-3752, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3750-3752.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • O'Hara, C. M. (2005). Manual and Automated Instrumentation for Identification of Enterobacteriaceae and Other Aerobic Gram-Negative Bacilli. Clin. Microbiol. Rev. 18: 147-162 [Abstract] [Full Text]  
  • Wauters, G., Avesani, V., Charlier, J., Janssens, M., Delmee, M. (2004). Histidine Decarboxylase in Enterobacteriaceae Revisited. J. Clin. Microbiol. 42: 5923-5924 [Abstract] [Full Text]  


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