This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peacock, S. J.
Right arrow Articles by Day, N. P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peacock, S. J.
Right arrow Articles by Day, N. P. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2002, p. 3764-3770, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3764-3770.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis as Tools for Typing Staphylococcus aureus Isolates in a Microepidemiological Setting

S. J. Peacock,1* G. D. I. de Silva,1,2 A. Justice,1 A. Cowland,1 C. E. Moore,3 C. G. Winearls,4 and N. P. J. Day3

Nuffield Department of Clinical Laboratory Sciences,1 Department of Pediatrics,2 Nuffield Department of Clinical Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU,3 Oxford Renal Unit, The Churchill, Oxford OX3 7LJ, United Kingdom4

Received 11 April 2002/ Returned for modification 27 May 2002/ Accepted 14 July 2002

Multilocus sequence typing (MLST) of Staphylococcus aureus is well suited to the study of global or long-term epidemiology, but its role in local epidemiology has not been defined. The present study has compared MLST with pulsed-field gel electrophoresis (PFGE) by using S. aureus isolates associated with carriage and disease in a busy regional renal unit. One hundred forty-four patients were prospectively recruited, of whom 103 were receiving hemodialysis and 41 were on continuous ambulatory peritoneal dialysis. Three nasal swab specimens were obtained 1 month apart on entering the study. A nasal swab was positive for S. aureus on at least one occasion in 50 patients (35%). Typing of the 104 carriage isolates demonstrated 21 PFGE types and 21 sequence types (STs). Thirty-one carriers had two or more positive nasal swabs; of these, the isolates in all swabs from a given carrier had identical PFGE types for 29 carriers; the isolates in all of the same 29 swabs had identical STs. The carriage strain in two patients changed both PFGE type and STs during the period of swabbing. Eight patients (6%) had an episode of S. aureus bacteremia during the 12-month study period, and two of these were nasal carriers. One of these invasive isolates had the same PFGE type and ST as the carriage isolate. There were no differences between Simpson's index of diversity for PFGE and Simpson's index of diversity for MLST for both invasive and carriage isolates, suggesting that the two methods have very similar discriminatory abilities. We conclude that PFGE and MLST performed equally in this study.

* Corresponding author. Mailing address: Department of Microbiology, Level 7, John Radcliffe Hospital, Headington, Oxford OX3 9DU, United Kingdom. Phone: 00-44-1865-220537. Fax: 00-44-1865-220984. E-mail: sharon.peacock{at}ndcls.ox.ac.uk.

Journal of Clinical Microbiology, October 2002, p. 3764-3770, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3764-3770.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sakwinska, O., Kuhn, G., Balmelli, C., Francioli, P., Giddey, M., Perreten, V., Riesen, A., Zysset, F., Blanc, D. S., Moreillon, P. (2009). Genetic Diversity and Ecological Success of Staphylococcus aureus Strains Colonizing Humans. Appl. Environ. Microbiol. 75: 175-183 [Abstract] [Full Text]  
  • Collery, M. M., Smyth, D. S., Twohig, J. M., Shore, A. C., Coleman, D. C., Smyth, C. J. (2008). Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis. J Med Microbiol 57: 348-358 [Abstract] [Full Text]  
  • Cai, Y., Kong, F., Wang, Q., Tong, Z., Sintchenko, V., Zeng, X., Gilbert, G. L. (2007). Comparison of Single- and Multilocus Sequence Typing and Toxin Gene Profiling for Characterization of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 3302-3308 [Abstract] [Full Text]  
  • Monk, A. B., Archer, G. L. (2007). Use of Outer Surface Protein Repeat Regions for Improved Genotyping of Staphylococcus epidermidis. J. Clin. Microbiol. 45: 730-735 [Abstract] [Full Text]  
  • Ghebremedhin, B., Konig, W., Witte, W., Hardy, K. J., Hawkey, P. M., Konig, B. (2007). Subtyping of ST22-MRSA-IV (Barnim epidemic MRSA strain) at a university clinic in Germany from 2002 to 2005. J Med Microbiol 56: 365-375 [Abstract] [Full Text]  
  • Hallin, M., Deplano, A., Denis, O., De Mendonca, R., De Ryck, R., Struelens, M. J. (2007). Validation of Pulsed-Field Gel Electrophoresis and spa Typing for Long-Term, Nationwide Epidemiological Surveillance Studies of Staphylococcus aureus Infections. J. Clin. Microbiol. 45: 127-133 [Abstract] [Full Text]  
  • Singh, A., Goering, R. V., Simjee, S., Foley, S. L., Zervos, M. J. (2006). Application of Molecular Techniques to the Study of Hospital Infection. Clin. Microbiol. Rev. 19: 512-530 [Abstract] [Full Text]  
  • Layer, F., Ghebremedhin, B., Konig, W., Konig, B. (2006). Heterogeneity of Methicillin-Susceptible Staphylococcus aureus Strains at a German University Hospital Implicates the Circulating-Strain Pool as a Potential Source of Emerging Methicillin-Resistant S. aureus Clones.. J. Clin. Microbiol. 44: 2179-2185 [Abstract] [Full Text]  
  • Ruiz-Garbajosa, P., Bonten, M. J. M., Robinson, D. A., Top, J., Nallapareddy, S. R., Torres, C., Coque, T. M., Canton, R., Baquero, F., Murray, B. E., del Campo, R., Willems, R. J. L. (2006). Multilocus Sequence Typing Scheme for Enterococcus faecalis Reveals Hospital-Adapted Genetic Complexes in a Background of High Rates of Recombination.. J. Clin. Microbiol. 44: 2220-2228 [Abstract] [Full Text]  
  • Durand, G., Bes, M., Meugnier, H., Enright, M. C., Forey, F., Liassine, N., Wenger, A., Kikuchi, K., Lina, G., Vandenesch, F., Etienne, J. (2006). Detection of New Methicillin-Resistant Staphylococcus aureus Clones Containing the Toxic Shock Syndrome Toxin 1 Gene Responsible for Hospital- and Community-Acquired Infections in France.. J. Clin. Microbiol. 44: 847-853 [Abstract] [Full Text]  
  • Koessler, T., Francois, P., Charbonnier, Y., Huyghe, A., Bento, M., Dharan, S., Renzi, G., Lew, D., Harbarth, S., Pittet, D., Schrenzel, J. (2006). Use of Oligoarrays for Characterization of Community-Onset Methicillin-Resistant Staphylococcus aureus.. J. Clin. Microbiol. 44: 1040-1048 [Abstract] [Full Text]  
  • Jorgensen, H. J., Mork, T., Caugant, D. A., Kearns, A., Rorvik, L. M. (2005). Genetic Variation among Staphylococcus aureus Strains from Norwegian Bulk Milk. Appl. Environ. Microbiol. 71: 8352-8361 [Abstract] [Full Text]  
  • Scally, M., Schuenzel, E. L., Stouthamer, R., Nunney, L. (2005). Multilocus Sequence Type System for the Plant Pathogen Xylella fastidiosa and Relative Contributions of Recombination and Point Mutation to Clonal Diversity. Appl. Environ. Microbiol. 71: 8491-8499 [Abstract] [Full Text]  
  • van der Zee, A., Heck, M., Sterks, M., Harpal, A., Spalburg, E., Kazobagora, L., Wannet, W. (2005). Recognition of SCCmec Types According to Typing Pattern Determined by Multienzyme Multiplex PCR-Amplified Fragment Length Polymorphism Analysis of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 43: 6042-6047 [Abstract] [Full Text]  
  • Wisplinghoff, H., Ewertz, B., Wisplinghoff, S., Stefanik, D., Plum, G., Perdreau-Remington, F., Seifert, H. (2005). Molecular Evolution of Methicillin-Resistant Staphylococcus aureus in the Metropolitan Area of Cologne, Germany, from 1984 to 1998. J. Clin. Microbiol. 43: 5445-5451 [Abstract] [Full Text]  
  • Berglund, C., Molling, P., Sjoberg, L., Soderquist, B. (2005). Multilocus Sequence Typing of Methicillin-Resistant Staphylococcus aureus from an Area of Low Endemicity by Real-Time PCR. J. Clin. Microbiol. 43: 4448-4454 [Abstract] [Full Text]  
  • Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E., Dowson, C. G. (2005). Multilocus Sequence Typing of Staphylococcus aureus Isolated from High-Somatic-Cell-Count Cows and the Environment of an Organic Dairy Farm in the United Kingdom. J. Clin. Microbiol. 43: 4731-4736 [Abstract] [Full Text]  
  • Nemoy, L. L., Kotetishvili, M., Tigno, J., Keefer-Norris, A., Harris, A. D., Perencevich, E. N., Johnson, J. A., Torpey, D., Sulakvelidze, A., Morris, J. G. Jr., Stine, O. C. (2005). Multilocus Sequence Typing versus Pulsed-Field Gel Electrophoresis for Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates. J. Clin. Microbiol. 43: 1776-1781 [Abstract] [Full Text]  
  • Shutt, C. K., Pounder, J. I., Page, S. R., Schaecher, B. J., Woods, G. L. (2005). Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive-Sequence-Based PCR for Characterization of Staphylococcus aureus Strains. J. Clin. Microbiol. 43: 1187-1192 [Abstract] [Full Text]  
  • Healy, M., Huong, J., Bittner, T., Lising, M., Frye, S., Raza, S., Schrock, R., Manry, J., Renwick, A., Nieto, R., Woods, C., Versalovic, J., Lupski, J. R. (2005). Microbial DNA Typing by Automated Repetitive-Sequence-Based PCR. J. Clin. Microbiol. 43: 199-207 [Abstract] [Full Text]  
  • Perez-Roth, E., Lorenzo-Diaz, F., Batista, N., Moreno, A., Mendez-Alvarez, S. (2004). Tracking Methicillin-Resistant Staphylococcus aureus Clones during a 5-Year Period (1998 to 2002) in a Spanish Hospital. J. Clin. Microbiol. 42: 4649-4656 [Abstract] [Full Text]  
  • Coombs, G. W., Nimmo, G. R., Bell, J. M., Huygens, F., O'Brien, F. G., Malkowski, M. J., Pearson, J. C., Stephens, A. J., Giffard, P. M., the Australian Group for Antimicrobial Resistance, (2004). Genetic Diversity among Community Methicillin-Resistant Staphylococcus aureus Strains Causing Outpatient Infections in Australia. J. Clin. Microbiol. 42: 4735-4743 [Abstract] [Full Text]  
  • Koreen, L., Ramaswamy, S. V., Graviss, E. A., Naidich, S., Musser, J. M., Kreiswirth, B. N. (2004). spa Typing Method for Discriminating among Staphylococcus aureus Isolates: Implications for Use of a Single Marker To Detect Genetic Micro- and Macrovariation. J. Clin. Microbiol. 42: 792-799 [Abstract] [Full Text]  
  • Harmsen, D., Claus, H., Witte, W., Rothganger, J., Claus, H., Turnwald, D., Vogel, U. (2003). Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management. J. Clin. Microbiol. 41: 5442-5448 [Abstract] [Full Text]  
  • Peacock, S. J., Justice, A., Griffiths, D., de Silva, G. D. I., Kantzanou, M. N., Crook, D., Sleeman, K., Day, N. P. J. (2003). Determinants of Acquisition and Carriage of Staphylococcus aureus in Infancy. J. Clin. Microbiol. 41: 5718-5725 [Abstract] [Full Text]  
  • Diep, B. A., Perdreau-Remington, F., Sensabaugh, G. F. (2003). Clonal Characterization of Staphylococcus aureus by Multilocus Restriction Fragment Typing, a Rapid Screening Approach for Molecular Epidemiology. J. Clin. Microbiol. 41: 4559-4564 [Abstract] [Full Text]  
  • Aires de Sousa, M., Bartzavali, C., Spiliopoulou, I., Santos Sanches, I., Crisostomo, M. I., de Lencastre, H. (2003). Two International Methicillin-Resistant Staphylococcus aureus Clones Endemic in a University Hospital in Patras, Greece. J. Clin. Microbiol. 41: 2027-2032 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»