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Journal of Clinical Microbiology, October 2002, p. 3793-3797, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3793-3797.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of 16S rRNA Gene Sequencing for Identification of Nonfermenting Gram-Negative Bacilli Recovered from Patients Attending a Single Cystic Fibrosis Center

Agnes Ferroni,1* Isabelle Sermet-Gaudelus,2 Eric Abachin,1 Gilles Quesne,1 Gerard Lenoir,2 Patrick Berche,1 and Jean-Louis Gaillard3

Microbiology Laboratory,1 Department of Pediatrics, Hôpital Necker-Enfants Malades, Paris,2 Microbiology Laboratory, Hôpital Raymond Poincaré, Garches, France3

Received 18 March 2002/ Returned for modification 28 May 2002/ Accepted 14 July 2002

During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (1 isolate), Burkholderia gladioli (1 isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France. Phone: 33 1 44 49 49 61. Fax: 33 1 44 49 49 60. E-mail: agnes.ferroni{at}nck.ap-hop-paris.fr.

Journal of Clinical Microbiology, October 2002, p. 3793-3797, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3793-3797.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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