Previous Article | Next Article 
Journal of Clinical Microbiology, October 2002, p. 3793-3797, Vol. 40, No. 10
0095-1137/02/$04.00+0 DOI: 10.1128/JCM.40.10.3793-3797.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Use of 16S rRNA Gene Sequencing for Identification of Nonfermenting Gram-Negative Bacilli Recovered from Patients Attending a Single Cystic Fibrosis Center
Agnes Ferroni,1* Isabelle Sermet-Gaudelus,2 Eric Abachin,1 Gilles Quesne,1 Gerard Lenoir,2 Patrick Berche,1 and Jean-Louis Gaillard3
Microbiology Laboratory,1
Department of Pediatrics, Hôpital Necker-Enfants Malades, Paris,2
Microbiology Laboratory, Hôpital Raymond Poincaré, Garches, France3
Received 18 March 2002/
Returned for modification 28 May 2002/
Accepted 14 July 2002
During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (1 isolate), Burkholderia gladioli (1 isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.
* Corresponding author. Mailing address: Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France. Phone: 33 1 44 49 49 61. Fax: 33 1 44 49 49 60. E-mail:
agnes.ferroni{at}nck.ap-hop-paris.fr.
Journal of Clinical Microbiology, October 2002, p. 3793-3797, Vol. 40, No. 10
0095-1137/02/$04.00+0 DOI: 10.1128/JCM.40.10.3793-3797.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
-
Kidd, T. J., Ramsay, K. A., Hu, H., Bye, P. T. P., Elkins, M. R., Grimwood, K., Harbour, C., Marks, G. B., Nissen, M. D., Robinson, P. J., Rose, B. R., Sloots, T. P., Wainwright, C. E., Bell, S. C., and the ACPinCF Investigators,
(2009). Low Rates of Pseudomonas aeruginosa Misidentification in Isolates from Cystic Fibrosis Patients. J. Clin. Microbiol.
47: 1503-1509
[Abstract]
[Full Text]
-
Segonds, C., Clavel-Batut, P., Thouverez, M., Grenet, D., Le Coustumier, A., Plesiat, P., Chabanon, G.
(2009). Microbiological and Epidemiological Features of Clinical Respiratory Isolates of Burkholderia gladioli. J. Clin. Microbiol.
47: 1510-1516
[Abstract]
[Full Text]
-
Su, S. C., Vaneechoutte, M., Dijkshoorn, L., Wei, Y. F., Chen, Y. L., Chang, T. C.
(2009). Identification of non-fermenting Gram-negative bacteria of clinical importance by an oligonucleotide array. J Med Microbiol
58: 596-605
[Abstract]
[Full Text]
-
Degand, N., Carbonnelle, E., Dauphin, B., Beretti, J.-L., Le Bourgeois, M., Sermet-Gaudelus, I., Segonds, C., Berche, P., Nassif, X., Ferroni, A.
(2008). Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cystic Fibrosis Patients. J. Clin. Microbiol.
46: 3361-3367
[Abstract]
[Full Text]
-
Bosch, A., Minan, A., Vescina, C., Degrossi, J., Gatti, B., Montanaro, P., Messina, M., Franco, M., Vay, C., Schmitt, J., Naumann, D., Yantorno, O.
(2008). Fourier Transform Infrared Spectroscopy for Rapid Identification of Nonfermenting Gram-Negative Bacteria Isolated from Sputum Samples from Cystic Fibrosis Patients. J. Clin. Microbiol.
46: 2535-2546
[Abstract]
[Full Text]
-
Mellmann, A., Cloud, J., Maier, T., Keckevoet, U., Ramminger, I., Iwen, P., Dunn, J., Hall, G., Wilson, D., LaSala, P., Kostrzewa, M., Harmsen, D.
(2008). Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting Bacteria. J. Clin. Microbiol.
46: 1946-1954
[Abstract]
[Full Text]
-
Pimentel, J. D., Dubedat, S. M., N. Dodds, E. L., Benn, R. A. V.
(2007). Identification of Isolates within the Burkholderia cepacia Complex by a Multiplex recA and 16S rRNA Gene Real-Time PCR Assay. J. Clin. Microbiol.
45: 3853-3854
[Full Text]
-
Luna, R. A., Fasciano, L. R., Jones, S. C., Boyanton, B. L. Jr., Ton, T. T., Versalovic, J.
(2007). DNA Pyrosequencing-Based Bacterial Pathogen Identification in a Pediatric Hospital Setting. J. Clin. Microbiol.
45: 2985-2992
[Abstract]
[Full Text]
-
Simmon, K. E., Croft, A. C., Petti, C. A.
(2006). Application of SmartGene IDNS Software to Partial 16S rRNA Gene Sequences for a Diverse Group of Bacteria in a Clinical Laboratory. J. Clin. Microbiol.
44: 4400-4406
[Abstract]
[Full Text]
-
Wellinghausen, N., Wirths, B., Poppert, S.
(2006). Fluorescence In Situ Hybridization for Rapid Identification of Achromobacter xylosoxidans and Alcaligenes faecalis Recovered from Cystic Fibrosis Patients.. J. Clin. Microbiol.
44: 3415-3417
[Abstract]
[Full Text]
-
Bosshard, P. P., Zbinden, R., Abels, S., Boddinghaus, B., Altwegg, M., Bottger, E. C.
(2006). 16S rRNA Gene Sequencing versus the API 20 NE System and the VITEK 2 ID-GNB Card for Identification of Nonfermenting Gram-Negative Bacteria in the Clinical Laboratory. J. Clin. Microbiol.
44: 1359-1366
[Abstract]
[Full Text]
-
Wellinghausen, N., Kothe, J., Wirths, B., Sigge, A., Poppert, S.
(2005). Superiority of Molecular Techniques for Identification of Gram-Negative, Oxidase-Positive Rods, Including Morphologically Nontypical Pseudomonas aeruginosa, from Patients with Cystic Fibrosis. J. Clin. Microbiol.
43: 4070-4075
[Abstract]
[Full Text]
-
Van daele, S., Verhelst, R., Claeys, G., Verschraegen, G., Franckx, H., Van Simaey, L., de Ganck, C., De Baets, F., Vaneechoutte, M.
(2005). Shared Genotypes of Achromobacter xylosoxidans Strains Isolated from Patients at a Cystic Fibrosis Rehabilitation Center. J. Clin. Microbiol.
43: 2998-3002
[Abstract]
[Full Text]
-
Bosshard, P. P., Abels, S., Altwegg, M., Bottger, E. C., Zbinden, R.
(2004). Comparison of Conventional and Molecular Methods for Identification of Aerobic Catalase-Negative Gram-Positive Cocci in the Clinical Laboratory. J. Clin. Microbiol.
42: 2065-2073
[Abstract]
[Full Text]
-
Drancourt, M., Berger, P., Raoult, D.
(2004). Systematic 16S rRNA Gene Sequencing of Atypical Clinical Isolates Identified 27 New Bacterial Species Associated with Humans. J. Clin. Microbiol.
42: 2197-2202
[Abstract]
[Full Text]
-
Qin, X., Emerson, J., Stapp, J., Stapp, L., Abe, P., Burns, J. L.
(2003). Use of Real-Time PCR with Multiple Targets To Identify Pseudomonas aeruginosa and Other Nonfermenting Gram-Negative Bacilli from Patients with Cystic Fibrosis. J. Clin. Microbiol.
41: 4312-4317
[Abstract]
[Full Text]
-
Segonds, C., Paute, S., Chabanon, G.
(2003). Use of Amplified Ribosomal DNA Restriction Analysis for Identification of Ralstonia and Pandoraea Species: Interest in Determination of the Respiratory Bacterial Flora in Patients with Cystic Fibrosis. J. Clin. Microbiol.
41: 3415-3418
[Abstract]
[Full Text]