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Journal of Clinical Microbiology, October 2002, p. 3798-3801, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3798-3801.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Imipenem-EDTA Disk Method for Differentiation of Metallo-ß-Lactamase-Producing Clinical Isolates of Pseudomonas spp. and Acinetobacter spp.

Dongeun Yong,1 Kyungwon Lee,1,2* Jong Hwa Yum,2 Hee Bong Shin,1 Gian Maria Rossolini,3 and Yunsop Chong1

Department of Laboratory Medicine, Research Institute of Bacterial Resistance,1 Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea,2 Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita di Siena, I-53100 Siena, Italy3

Received 8 March 2002/ Returned for modification 16 May 2002/ Accepted 1 July 2002

Rapid detection of metallo-ß-lactamase (MBL)-producing gram-negative bacilli is necessary to prevent their dissemination. The method using a disk with imipenem plus 750 µg of EDTA differentiated all MBL-producing pseudomonads, and the sensitivity and specificity for acinetobacters were 95.7 and 91.0%, respectively. The imipenem-EDTA disks were stable for 12 and 16 weeks at 4 and -20°C, respectively.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemoon-ku, Seoul 120-752, Korea. Phone: 82-2-361-5866. Fax: 82-2-313-0908. E-mail: leekcp{at}yumc.yonsei.ac.kr.

Journal of Clinical Microbiology, October 2002, p. 3798-3801, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3798-3801.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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