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Journal of Clinical Microbiology, October 2002, p. 3802-3804, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3802-3804.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PCR Detection of Borrelia burgdorferi Sensu Lato, Tick-Borne Encephalitis Virus, and the Human Granulocytic Ehrlichiosis Agent in Ixodes persulcatus Ticks from Western Siberia, Russia

Olga V. Morozova,1,2 Andrey K. Dobrotvorsky,3 Natalya N. Livanova,3 Sergey E. Tkachev,1 Valentina N. Bakhvalova,3 Anatoly B. Beklemishev,4 and Felipe C. Cabello2*

Novosibirsk Institute of Bioorganic Chemistry,1 Institute of Systematics and Ecology of Animals,3 Institute of Biochemistry, Novosibirsk 630090, Russia,4 Department of Microbiology and Immunology, New York Medical College, Valhalla, New York 105952

Received 14 February 2002/ Returned for modification 9 June 2002/ Accepted 25 July 2002

PCR assays were used to test adult Ixodes persulcatus ticks from Western Siberia, Russia, for Borrelia burgdorferi sensu lato, tick-borne encephalitis virus (TBEV), and the human granulocytic ehrlichiosis (HGE) agent. Of the 150 ticks that were studied, 38% were infected with B. burgdorferi, 46% were infected with TBEV, and 8% were infected with the HGE agent. These three pathogens were distributed in the ticks independently of one another.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595. Phone: (914) 594-4182. Fax: (914) 594-4176. E-mail: cabello{at}nymc.edu.

Journal of Clinical Microbiology, October 2002, p. 3802-3804, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3802-3804.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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