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Journal of Clinical Microbiology, October 2002, p. 3810-3813, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3810-3813.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of embB306 Mutations in Ethambutol-Susceptible Clinical Isolates of Mycobacterium tuberculosis from Northwestern Russia: Implications for Genotypic Resistance Testing

Igor Mokrousov,1* Tatiana Otten,2 Boris Vyshnevskiy,2 and Olga Narvskaya1

Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, 197101 St. Petersburg,1 Laboratory of Microbiology of Tuberculosis, The Research Institute of Phthisiopulmonology, 193036 St. Petersburg, Russia2

Received 3 April 2002/ Returned for modification 9 June 2002/ Accepted 12 July 2002

A total of 183 epidemiologically unlinked Mycobacterium tuberculosis isolates collected in the St. Petersburg area of Russia from 1996 to 2001 were screened for alterations in codon 306 of the embB gene; mutations in this codon are reported to confer resistance to ethambutol (EMB). The embB306 mutations were detected in 14 (48.3%) of 29 EMB-resistant strains and, quite surprisingly, in 48 (31.2%) of 154 EMB-susceptible strains. A discrepancy between the results of phenotypic and genotypic EMB resistance tests was restricted to the strains already resistant to other antitubercular (anti-TB) drugs. In particular, 40 (60%) of the 69 EMB-susceptible strains resistant to rifampin, isoniazid, and streptomycin but none of the 43 pansusceptible strains harbored an embB306 mutation. We hypothesize that the phenomenon observed could reflect the presence of a target other than EmbB for the drug in tubercle bacilli; this unknown target could be sensitized and affected, sensu lato, by EMB during treatment with other first-line anti-TB drugs. Comparison with DNA fingerprinting data showed that, irrespectively of the phenotypic susceptibility profiles, 46 (50.6%) of 91 Beijing family strains and 16 (17.4%) of 92 strains of other genotypes had a mutation in embB306.

* Corresponding author. Mailing address: Pasteur Institute, 14 Mira St., 197101 St. Petersburg, Russia. Phone: 7 812 233 21 49. Fax: 7. 812 232 92 17. E-mail: miv{at}IM4520.spb.edu.

Journal of Clinical Microbiology, October 2002, p. 3810-3813, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3810-3813.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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