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Journal of Clinical Microbiology, October 2002, p. 3814-3817, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3814-3817.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila in Clinical Specimens by Dual-Color Real-Time PCR and Melting Curve Analysis

Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg,¹ TIB Molbiol GmbH, Tempelhofer Weg 11-12, D-10829 Berlin,² Institute of Medical Microbiology and Hygiene, University of Ulm, D-89081 Ulm, Germany,⁴ Health Waikato Laboratory, Hamilton, New Zealand³

Received 6 September 2001/ Returned for modification 28 May 2002/ Accepted 23 July 2002

A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory specimens from patients with atypical pneumonia, 100% sensitivity and specificity was observed for L. pneumophila.

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