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Journal of Clinical Microbiology, October 2002, p. 3838-3840, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3838-3840.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Impact of Nasopharyngeal Swab Types on Detection of Bordetella pertussis by PCR and Culture

Joann L. Cloud,1* Weston Hymas,2 and Karen C. Carroll1,3

Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology,1 ARUP Molecular Infectious Diseases Laboratory,2 Department of Pathology, University of Utah, Salt Lake City, Utah3

Received 24 April 2002/ Returned for modification 2 June 2002/ Accepted 13 July 2002

A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis. Dacron and rayon swabs are an excellent choice for both PCR and culture.

* Corresponding author. Mailing address: ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787, ext. 2439. Fax: (801) 584-5109. E-mail: cloudjl{at}aruplab.com.

Journal of Clinical Microbiology, October 2002, p. 3838-3840, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3838-3840.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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