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Journal of Clinical Microbiology, October 2002, p. 3841-3844, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3841-3844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparison of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing Proposed Standard and the E-Test with the NCCLS Broth Microdilution Method for Voriconazole and Caspofungin Susceptibility Testing of Yeast Species

Erja Chryssanthou1* and Manuel Cuenca-Estrella2

Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden,1 Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Spain2

Received 25 February 2002/ Returned for modification 8 May 2002/ Accepted 28 June 2002

The proposed standard of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing (AFST-EUCAST) and the E-test procedures were compared with the NCCLS reference broth microdilution method for voriconazole and caspofungin susceptibility testing of 102 clinical Candida species and Saccharomyces cerevisiae isolates. The voriconazole MIC at which 50% of strains were inhibited (MIC50) was <=0.125 mg/liter for all yeast species except for Candida glabrata and Candida krusei, which yielded MIC50 values of 0.25 to 1 mg/liter depending on the method. Caspofungin exhibited in vitro activity (MIC50 of <=0.125 to 2 mg/liter) against all yeast species except for Candida guilliermondii. The agreements between MICs within ±2 dilutions obtained by the NCCLS method and the EUCAST standard were 97% for voriconazole and 96% for caspofungin. Intraclass correlation coefficients were statistically significant (P < 0.05). The agreements between voriconazole MICs provided by the E-test and the NCCLS and between the E-test and the AFST-EUCAST method were 100 and 90%, respectively. Because of lower caspofungin MICs provided by the E-test, the agreement was slightly poorer with the NCCLS method (89%) than with the AFST-EUCAST procedure (94%). Both the EUCAST and the E-test procedures can be reliable techniques for susceptibility testing of yeasts to voriconazole and caspofungin.

* Corresponding author. Mailing address: Department of Clinical Microbiology, L202 Karolinska Hospital, S-171 76 Stockholm, Sweden. Phone: 46 851773566. Fax: 46 8308099. E-mail: erja.chryssanthou{at}ks.se.

Journal of Clinical Microbiology, October 2002, p. 3841-3844, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3841-3844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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