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Journal of Clinical Microbiology, October 2002, p. 3845-3847, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3845-3847.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Reduction of Contamination of Mycobacterial Growth Indicator Tubes with a Modified Antimicrobial Combination

Chulhun Ludgerus Chang,1* Tae Sung Park,1 Seung Hwan Oh,1 Hyung Hoi Kim,1 Eun Yup Lee,1 Han Chul Son,1 and Cheol Min Kim2

Departments of Clinical Pathology,1 Biochemistry, College of Medicine, Pusan National University, Pusan, Korea2

Received 12 April 2002/ Returned for modification 24 May 2002/ Accepted 8 July 2002

Culture in the fluorimetric Mycobacteria Growth Indicator Tube (MGIT) treated with a combination of vancomycin, amphotericin B, and nalidixic acid (VAN) showed growth of most strains of 31 mycobacterial species with a less-than-1-day delay. The results were similar to those in the MGIT with polymyxin B, amphotericin B, nalidixic acid, trimethoprim, and azlocillin, but with respiratory specimens, the MGIT with VAN showed a lower contamination rate with no change in the detection rate or time.

* Corresponding author. Mailing address: Department of Clinical Pathology, College of Medicine, Pusan National University, #10 1-Ga Ami-Dong Seo-Gu, Busan 602-739, Korea. Phone: 82-51-240-7418. Fax: 82-51-247-6560. E-mail: cchl{at}pusan.ac.kr.

Journal of Clinical Microbiology, October 2002, p. 3845-3847, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3845-3847.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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