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Journal of Clinical Microbiology, October 2002, p. 3857-3859, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3857-3859.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Detection of Human Calicivirus among Spanish Children with Acute Gastroenteritis

E. Roman,1 A. Negredo,2 R. M. Dalton,2 I. Wilhelmi,3 and A. Sánchez-Fauquier2*

Servicio de Pediatría,1 Servicio de Microbiología, Hospital Severo Ochoa, Leganés,3 Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain2

Received 24 May 2002/ Returned for modification 26 June 2002/ Accepted 17 July 2002

A survey was conducted among Spanish children with gastroenteritis treated in an emergency room. Reverse transcription-PCR with specimens negative for other enteric pathogens was used. The minimum incidence of human calicivirus infection was 7.7%, with Lordsdale as the predominant genotype. The clinical features and severity of calicivirus and rotavirus were similar.

* Corresponding author. Mailing address: Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda a Pozuelo Km. 2, 28220 Madrid, Spain. Phone: 34 91 509 79 01, ext. 3655. Fax: 34 91 509 79 66. E-mail: asanchez{at}isciii.es.

Journal of Clinical Microbiology, October 2002, p. 3857-3859, Vol. 40, No. 10
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.10.3857-3859.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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