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Journal of Clinical Microbiology, November 2002, p. 3913-3916, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3913-3916.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Alafosfalin as a Selective Agent for Isolation of Salmonella from Clinical Samples

J. D. Perry,1 G. Riley,1 F. K. Gould,1 J. M. Perez,2 E. Boissier,3 R. T. Ouedraogo,3 and A. M. Freydière4*

Department of Microbiology, Freeman Hospital, Newcastle upon Tyne, United Kingdom,1 Hôpital de Pointe-à-Pitre, Guadeloupe,2 Centre Hospitalier National Yalgado Ouedraogo, Burkina Faso,3 Laboratoire de Bactériologie, Hôpital Debrousse, F-69322 Lyon cedex 05, France4

Received 18 April 2002/ Returned for modification 27 June 2002/ Accepted 31 July 2002

The selectivity of a range of culture media for the detection of Salmonella was assessed using 435 strains of gram-negative bacteria. These media showed limited ability to inhibit non-Salmonella strains found in stool samples. We report the evaluation of alafosfalin as a selective agent for isolation of Salmonella from stool samples. Susceptibility studies with this agent showed that non-typhi Salmonella strains were relatively resistant (mean MIC, 10.2 mg/liter) compared to many coliforms including Escherichia coli (mean MIC, 0.7 mg/liter). A chromogenic medium, ABC medium, was modified to incorporate alafosfalin and was compared with standard ABC medium and Hektoen enteric agar for the isolation of Salmonella from 1,000 stool samples. On direct culture, modified ABC medium showed higher recovery of Salmonella (53.6%) compared with either ABC medium (35.7%) or Hektoen enteric agar (48.2%). We conclude that alafosfalin is a useful selective agent for the isolation of Salmonella from stool samples.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Hôpital Debrousse, Hospices Civils de Lyon, 29 Rue Soeur Bouvier, F-69322 Lyon cedex 05, France. Phone: (33) 4 72 38 58 16. Fax: (33) 4 72 38 55 35. E-mail: am.freydiere{at}chu-lyon.fr.

Journal of Clinical Microbiology, November 2002, p. 3913-3916, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3913-3916.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Perez, J. M., Cavalli, P., Roure, C., Renac, R., Gille, Y., Freydiere, A. M. (2003). Comparison of Four Chromogenic Media and Hektoen Agar for Detection and Presumptive Identification of Salmonella Strains in Human Stools. J. Clin. Microbiol. 41: 1130-1134 [Abstract] [Full Text]  


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