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Journal of Clinical Microbiology, November 2002, p. 3951-3955, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3951-3955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Both Urinary and Rectal Escherichia coli Isolates Are Dominated by Strains of Phylogenetic Group B2

Lixin Zhang,* Betsy Foxman, and Carl Marrs

Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, Michigan

Received 27 March 2002/ Returned for modification 25 May 2002/ Accepted 6 August 2002

To compare the genetic structures of uropathogenic and commensal Escherichia coli populations, a total of 181 urinary and rectal E. coli isolates were classified into intraspecies phylogenetic groups by PCR amplifications of phylogenetic markers. The genetic variability of these isolates within phylogenetic groups was further assessed by enterobacterial repetitive intergenic consensus (ERIC) typing. The distributions of 10 known virulence factors were also examined. In contrast with most reports, phylogenetic group B2 not only accounted for the majority of urinary isolates from young women with urinary tract infections (69%) but also was the dominant group among the rectal isolates from healthy young women (48%). Such difference may be explained by geographic variation, difference in host population characteristics, or differences in sampling method, or a combination of the three. Strains with known virulence factors most frequently belonged to phylogenetic groups B2 and D. Additionally, group B2 and D rectal isolates were more heterogeneous than urinary isolates. Two subclusters existed within group B2 strains by ERIC typing. These subclusters were not evenly distributed between rectal and urine isolates and differed in virulence gene distribution.

* Corresponding author. Mailing address: Department of Epidemiology, 109 Observatory St., Ann Arbor, MI 48109-2029. Phone: (734) 615-2775. Fax: (734) 936-6732. E-mail: lxzhang{at}umich.edu.

Journal of Clinical Microbiology, November 2002, p. 3951-3955, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3951-3955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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