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Journal of Clinical Microbiology, November 2002, p. 3999-4003, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3999-4003.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Collaborative Evaluation of Optimal Antifungal Susceptibility Testing Conditions for Dermatophytes

Belkys Fernández-Torres,1 Francisco J. Cabañes,2 Alfonso J. Carrillo-Muñoz,3 Alexandre Esteban,2 Isabel Inza,1 Lourdes Abarca,2 and Josep Guarro1*

Unitat de Microbiología, Facultat de Medicina, Universitat Rovira i Virgili, Reus,1 Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Bellaterra,2 Departament of Microbiología, Asesoría Científica y de Investigación Aplicada, Barcelona, Spain3

Received 13 March 2002/ Returned for modification 5 April 2002/ Accepted 18 August 2002

A multicenter study was conducted to define the most suitable testing conditions for antifungal susceptibility of dermatophytes. Broth microdilution MICs of clotrimazole, itraconazole, and terbinafine were determined in three centers against 60 strains of dermatophytes. The effects of inoculum density (ca. 103 and 104 CFU/ml), incubation time (3, 7, and 14 days), endpoint criteria for MIC determination (complete [MIC-0] and prominent [MIC-2] growth inhibition), and incubation temperature (28 and 37°C) on intra- and interlaboratory agreement were analyzed. The optimal testing conditions identified were an inoculum of 104 CFU/ml, a temperature of incubation of 28°C, an incubation period of 7 days, and MIC-0.

* Corresponding author. Mailing address: Unitat de Microbiologia, Facultat de Medicina, Universitat Rovira i Virgili, Carrer Sant Llorenç, 21, 43201 Reus, Spain. Phone: 34-977-759359. Fax: 34-977-759322. E-mail: umb{at}astor.urv.es.

Journal of Clinical Microbiology, November 2002, p. 3999-4003, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.3999-4003.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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