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Journal of Clinical Microbiology, November 2002, p. 4010-4014, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4010-4014.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Detection and Seroepidemiology of the Chlamydia pneumoniae Bacteriophage ({Phi}Cpn1)

Karuna P. Karunakaran,1 James F. Blanchard,2 Ausra Raudonikiene,3 Caixia Shen,1 Andrew D. Murdin,3 and Robert C. Brunham1*

University of British Columbia Centre for Disease Control, Vancouver,1 Department of Community Health Sciences and Department of Medical Microbiology, University of Manitoba, Winnipeg,2 Aventis Pasteur, Toronto, Canada3

Received 22 March 2002/ Returned for modification 29 May 2002/ Accepted 19 August 2002

Recent whole-genome analysis has demonstrated limited genetic variation in Chlamydia pneumoniae, with one strain (AR39) containing a 4,524 nucleotide single-stranded DNA bacteriophage, {Phi}Cpn1. Using PCR, reverse transcription (RT)-PCR, and Western blotting, we confirmed the presence and functional expression of {Phi}Cpn1 in C. pneumoniae strain AR39 and its absence in strain CWL029. Six additional epidemiologically distinct clinical isolates of C. pneumoniae also did not contain {Phi}Cpn1. We generated recombinant viral protein 1 (Vp1) from {Phi}Cpn1 in Escherichia coli and showed that Vp1 antigen is highly immunogenic in mice and that murine antisera readily recognize native Vp1 from C. pneumoniae strain AR39 elementary bodies (EB). We developed an enzyme-linked immunosorbent assay (ELISA) to measure antibodies to recombinant Vp1 in human sera collected from 32 patients with abdominal aortic aneurysm (AAA) and 40 controls. Among the 72 subjects, 61 had C. pneumoniae EB antibodies shown by ELISA. Antibodies to Vp1 were found in 39 of the 61 (64%) seropositive individuals and were significantly correlated with AAA (adjusted odds ratio, 13.9; 95% confidence interval, 1.1 to 175). Our studies indicate that phage-containing strains of C. pneumoniae are uncommonly found by isolation but may commonly infect individuals with vascular disease.

* Corresponding author. Mailing address: UBC Centre for Disease Control, 655 West 12th Ave., Vancouver, BC V5Z 4R4, Canada. Phone: (604) 660-2626. Fax: (604) 660-6066. E-mail: robert.brunham@bccdc.ca.

Journal of Clinical Microbiology, November 2002, p. 4010-4014, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4010-4014.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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