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Journal of Clinical Microbiology, November 2002, p. 4030-4036, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4030-4036.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification and Broad Dissemination of the CTX-M-14 ß-Lactamase in Different Escherichia coli Strains in the Northwest Area of Spain

German Bou,* Monica Cartelle, Maria Tomas, Delia Canle, Francisca Molina, Rita Moure, Jose Maria Eiros, and Antonio Guerrero{dagger}

Servicio de Microbiología, Complejo Hospitalario Juan Canalejo, 15006 La Coruña, Spain

Received 22 April 2002/ Returned for modification 2 June 2002/ Accepted 5 August 2002

During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 ß-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spectrum ß-lactamase were collected from January to October 2001 and studied for the presence of the CTX-M-14 ß- lactamase gene. By isoelectric point determination, PCR, and nucleotide sequencing, we detected the presence of this gene in 17 E. coli strains belonging to 15 different genotypes (REP-PCR) causing infections in 17 different patients. Epidemiological studies based on medical records did not suggest any relationship between the patients infected with these E. coli strains and, interestingly, 7 of 30 patients harboring strains with extended-spectrum ß-lactamases never had contact with the hospital environment before the clinical E. coli isolation. Conjugation experiments revealed that this gene was plasmid mediated in the 17 E. coli strains, and plasmid restriction fragment length polymorphisms showed 9 different patterns in the 17 E. coli strains. By PCR, the sequence of the tnpA transposase gene of the insert sequence ISEcp-1 was detected in all the plasmids harboring the CTX-M-14 gene. These results strongly suggest that plasmid dissemination between different E. coli strains in addition to a mobile element (transposon) around the ß-lactamase gene may be involved in the spreading of the CTX-M-14 gene. This study reinforces the hypothesis that the epidemiology of the prevalence of the ß-lactamase genes is changing and should alert the medical community to the increase in the emergence of the CTX-M ß-lactamases worldwide.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Materno Infantil, Complejo Hospitalario Juan Canalejo, C/Xubias de Arriba 84, 15006 La Coruña, Spain. Phone: 34-981-178000, ext. 21171. Fax: 34-981-178216. E-mail: germanbou{at}canalejo.org.

{dagger} Present address: Hospital de la Ribera, Alzira, Valencia, Spain.

Journal of Clinical Microbiology, November 2002, p. 4030-4036, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4030-4036.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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