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Journal of Clinical Microbiology, November 2002, p. 4051-4055, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4051-4055.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Nosocomial Infections Caused by Multidrug-Resistant Isolates of Pseudomonas putida Producing VIM-1 Metallo-ß-Lactamase

Gianluigi Lombardi,1 Francesco Luzzaro,1 Jean-Denis Docquier,2 Maria Letizia Riccio,2 Mariagrazia Perilli,3 Alessandra Colì,1 Gianfranco Amicosante,3 Gian Maria Rossolini,2* and Antonio Toniolo1*

Laboratorio di Microbiologia, Ospedale di Circolo, and Università dell'Insubria, I-21100 Varese,1 Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena,2 Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, I-67100 L'Aquila, Italy3

Received 21 May 2002/ Returned for modification 7 July 2002/ Accepted 12 August 2002

Successful carbapenem-based chemotherapy for the treatment of Pseudomonas infections has been seriously hindered by the recent appearance of IMP- and VIM-type metallo-ß-lactamases, which confer high-level resistance to carbapenems and most other ß-lactams. Recently, multidrug-resistant Pseudomonas putida isolates for which carbapenem MICs were >=32 µg/ml were recovered from cultures of urine from three inpatients in the general intensive care unit of the Ospedale di Circolo, Varese, Italy. Enzyme assays revealed production of a metallo-ß-lactamase activity, while molecular analysis detected in each isolate a blaVIM-1 determinant carried by an apparently identical medium-sized plasmid. Conjugation experiments were unsuccessful in transferring the ß-lactamase determinant to Escherichia coli or Pseudomonas aeruginosa. Macrorestriction analysis by pulsed-field gel electrophoresis demonstrated that the isolates were of clonal origin. PCR mapping and sequencing of the variable region of the plasmid-borne class 1 integron carrying the blaVIM-1 determinant (named In110) showed that the blaVIM-1-containing cassette was identical to that previously found in strains of different species from other Italian hospitals and that the cassette array of In110 was not identical but clearly related to that of In70 (a blaVIM-1-containing plasmid-borne integron from an Achromobacter xylosoxidans isolate), pointing to a common origin of this cassette and to a related evolutionary history of their cognate integrons.

* Corresponding author. Mailing address for Gian Maria Rossolini: Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Policlinico Le Scotte, 53100 Siena, Italy. Phone: 39-0577-233327. Fax: 39-0577-233325. E-mail: rossolini{at}unisi.it. Mailing address for Antonio Toniolo: Laboratorio di Microbiologia, Ospedale di Circolo e Università dell'Insubria, Viale Borri 57, 21100 Varese, Italy. Phone: 39-0332-278309. Fax: 39-0332-260820. E-mail: antonio.toniolo{at}ospedale.varese.it.

Journal of Clinical Microbiology, November 2002, p. 4051-4055, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4051-4055.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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