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Journal of Clinical Microbiology, November 2002, p. 4138-4142, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4138-4142.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Performance Assessment of Two Commercial Amplification Assays for Direct Detection of Mycobacterium tuberculosis Complex from Respiratory and Extrapulmonary Specimens

Claudio Piersimoni,1* Claudio Scarparo,2 Paola Piccoli,2 Alessandra Rigon,2 Giuliana Ruggiero,2 Domenico Nista,1 and Stefano Bornigia1

Department of Clinical Microbiology, General Hospital Umberto I-Torrette, Ancona,1 Regional Mycobacteria Reference Centre, San Bortolo Hospital, Vicenza, Italy2

Received 11 February 2002/ Returned for modification 30 June 2002/ Accepted 16 August 2002

The new BDProbeTec ET Mycobacterium tuberculosis Complex Direct Detection Assay (DTB) was compared with the enhanced M. tuberculosis Amplified Direct Test (AMTDII). The system is an automated walkaway system characterized by simultaneous DNA amplification (strand displacement amplification) and real-time fluorometric detection. It also contains an internal amplification control (IAC) designed to identify inhibition from the processed samples. The AMTDII assay amplifies rRNA by transcription-mediated amplification; it uses hybridization with a chemoluminescent probe as a detection system and is entirely manual. A total of 515 N-acetyl-L-cysteine-sodium hydroxide-decontaminated respiratory (n = 331) and extrapulmonary (n = 184) sediments (from 402 patients) were tested in parallel by both assays. The results were compared with those of acid-fast staining and culture (solid plus liquid media), setting the combination of culture and clinical diagnosis as the "gold standard." Culture results from the tested specimens were as follows: 121 Mycobacterium tuberculosis complex (MTB) (98 smear-positive), 46 nontuberculous mycobacteria (38 smear-positive), and 338 culture-negative results. After resolution of the discrepant results, the percent sensitivity, percent specificity, and positive and negative likelihood ratios for AMTDII were 88%, 99.2%, 110, and 0.11 for respiratory specimens and 74.3%, 100%, 740, and 0.26 for extrapulmonary specimens, respectively. The corresponding values for DTB were 94.5%, 99.6%, 235, and 0.05 for respiratory specimens and 92.3%, 100%, 920, and 0.07 for extrapulmonary specimens, respectively. The cumulative difference for all tuberculosis-positive extrapulmonary specimens was significant (P = 0.03). The overall inhibition rate for DTB was 5% (26 specimens). We conclude that both amplification assays proved to be rapid and specific for the detection of MTB in clinical samples and particularly feasible for a routine laboratory work flow. DTB combines a labor-intensive specimen preparation procedure with a completely automated amplification and detection. Finally, differences between AMTDII and DTB sensitivities were associated with the presence of inhibitory samples that the former assay, lacking IAC, could not detect.

* Corresponding author. Mailing address: Department of Clinical Microbiology, General Hospital Umberto I-Torrette, Via Conca, Ancona I-60020, Italy. Phone: 39-071-596-3049. Fax: 39-071-596-4184. E-mail: piersim{at}tin.it.

Journal of Clinical Microbiology, November 2002, p. 4138-4142, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4138-4142.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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