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Journal of Clinical Microbiology, November 2002, p. 4295-4297, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4295-4297.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Direct Detection and Identification of African Trypanosomes by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes

M. Radwanska,1* S. Magez,2 H. Perry-O'Keefe,3 H. Stender,3 J. Coull,3 J. M. Sternberg,4 P. Büscher,1 and J. J. Hyldig-Nielsen3

Department of Parasitology, Institute of Tropical Medicine, Antwerp,1 Laboratory of Cellular Immunology, Flanders Interuniversity Institute for Biotechnology, Free University of Brussels VUB, Brussels, Belgium,2 Applied Biosystems, Bedford, Massachusetts,3 University of Aberdeen, Aberdeen, Scotland4

Received 19 April 2002/ Returned for modification 18 June 2002/ Accepted 24 July 2002

We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

* Corresponding author. Present address: Department of Immunology, Groote Schuur Hospital, Old Main Building H47, Observatory 7925, Cape Town, South Africa. Phone: 27 21 404 4013. Fax: 27 21 448 6116. E-mail: mradwans{at}uctgsh1.uct.ac.za.

Journal of Clinical Microbiology, November 2002, p. 4295-4297, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4295-4297.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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