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Journal of Clinical Microbiology, November 2002, p. 4298-4300, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4298-4300.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection of Oxazolidinone-Resistant Enterococcus faecalis and Enterococcus faecium Strains by Real-Time PCR and PCR-Restriction Fragment Length Polymorphism Analysis

Neil Woodford,1* Luke Tysall,1 Cressida Auckland,2 Mark W. Stockdale,1 Andrew J. Lawson,3 Rachel A. Walker,1 and David M. Livermore1

Antibiotic Resistance Monitoring and Reference Laboratory,1 Laboratory of Enteric Pathogens, Central Public Health Laboratory, London NW9 5HT,3 Southampton Public Health Laboratory, Southampton SO16 6YD, United Kingdom2

Received 23 May 2002/ Returned for modification 7 July 2002/ Accepted 5 August 2002

A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management.

* Corresponding author. Mailing address: ARMRL, CPHL, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44-20-8200-4400. Fax: 44-20-8358-3292. E-mail: nwoodford{at}phls.org.uk.

Journal of Clinical Microbiology, November 2002, p. 4298-4300, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4298-4300.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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