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Journal of Clinical Microbiology, November 2002, p. 4301-4303, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4301-4303.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cryopreserved Cell Monolayers for Rapid Detection of Herpes Simplex Virus and Influenza Virus

Yung T. Huang,1* Huimin Yan,1 Yan Sun,1 Joseph A. Jollick, Jr.,2 and Heather Baird2

Department of Pathology, University Hospitals of Cleveland, Case Western Reserve University, Cleveland, Ohio 44106,1 Diagnostic Hybrids Inc., Athens, Ohio 457012

Received 15 March 2002/ Returned for modification 15 June 2002/ Accepted 17 August 2002

Cryopreserved cell monolayers are a new cell culture technology intended to ensure the availability of cells in the laboratory for virus detection. Two cryopreserved cell monolayers, ELVIS for the detection of herpes simplex virus (HSV) and R-Mix for the detection of influenza virus, were evaluated. The results indicated that fresh and cryopreserved cell monolayers are comparable in sensitivity for the detection of HSV and influenza virus. The cells retain the same level of sensitivity for up to 4 months at -80°C.

* Corresponding author. Mailing address: Department of Pathology, University Hospitals of Cleveland, Case Western Reserve University, 2085 Adelbert Rd., Cleveland, OH 44106. Phone: (216) 844-8611. Fax: (216) 844-5601. E-mail: yth{at}po.cwru.edu.

Journal of Clinical Microbiology, November 2002, p. 4301-4303, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4301-4303.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2002 by the American Society for Microbiology. All rights reserved.