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Journal of Clinical Microbiology, November 2002, p. 4304-4307, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4304-4307.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria

Sven Klaschik,* Lutz E. Lehmann, Ansgar Raadts, Malte Book, Andreas Hoeft, and Frank Stuber

Department of Anesthesiology and Intensive Care Medicine, University of Bonn, Bonn, Germany

Received 29 April 2002/ Returned for modification 14 July 2002/ Accepted 17 August 2002

We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes. All tested bacteria were identified correctly, and none gave a false-positive signal with the opposite Gram probe.

* Corresponding author. Mailing address: Department of Anesthesiology and Intensive Care Medicine, University of Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany. Phone: 49-228-287-6018. Fax: 49-228-287-6754. E-mail: klaschik{at}ukb.uni-bonn.de.

Journal of Clinical Microbiology, November 2002, p. 4304-4307, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4304-4307.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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