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Journal of Clinical Microbiology, November 2002, p. 4317-4320, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4317-4320.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Serological Differentiation of Infections with Dengue Virus Serotypes 1 to 4 by Using Recombinant Antigens

Diana Ludolfs, Stefan Schilling, Jan Altenschmidt, and Herbert Schmitz*

Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany

Received 21 February 2002/ Returned for modification 5 May 2002/ Accepted 4 August 2002

The B domains of dengue virus serotypes 1 to 4 were expressed in Escherichia coli. The purified proteins were applied to immunoblot strips to detect serotype-specific antibodies in paired serum samples from 41 patients with primary and secondary dengue infections. A close correlation between the results obtained with the immunoblot strips and by type-specific reverse transcription-PCR (T. Laue, P. Emmerich, and H. Schmitz, J. Clin. Microbiol. 37:2543-2547, 1999) was observed.

* Corresponding author. Mailing address: Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, D-20359 Hamburg, Germany. Phone: 49-40-42818-460. Fax: 49-40-42818-378. E-mail: schmitz{at}bni.uni-hamburg.de.

Journal of Clinical Microbiology, November 2002, p. 4317-4320, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4317-4320.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology. All rights reserved.