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Journal of Clinical Microbiology, November 2002, p. 4321-4324, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4321-4324.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Development of a Hexaplex PCR Assay for Rapid Detection of Virulence and Regulatory Genes in Vibrio cholerae and Vibrio mimicus

Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram 695 014, Kerala, India,¹ Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202,² Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742³

Received 2 May 2002/ Returned for modification 18 June 2002/ Accepted 20 August 2002

We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in Vibrio cholerae and Vibrio mimicus. This hexaplex PCR proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic V. cholerae and V. mimicus strains from both clinical and environmental sources.

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