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Journal of Clinical Microbiology, November 2002, p. 4321-4324, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4321-4324.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Development of a Hexaplex PCR Assay for Rapid Detection of Virulence and Regulatory Genes in Vibrio cholerae and Vibrio mimicus

D. V. Singh,1* Sree Renjini Isac,1 and R. R. Colwell2,3

Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram 695 014, Kerala, India,1 Center for Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202,2 Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 207423

Received 2 May 2002/ Returned for modification 18 June 2002/ Accepted 20 August 2002

We have developed a hexaplex PCR assay for rapid detection of the virulence and regulatory genes for cholera toxin enzymatic subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), toxin-coregulated pilus (tcpA), outer membrane protein (ompU), and central regulatory protein ToxR (toxR) in Vibrio cholerae and Vibrio mimicus. This hexaplex PCR proved successful in screening pathogenic-toxigenic and nonpathogenic-nontoxigenic V. cholerae and V. mimicus strains from both clinical and environmental sources.

* Corresponding author. Mailing address: Rajiv Gandhi Centre for Biotechnology, Thycaud P.O., Poojappura, Thiruvananthapuram 695 014, Kerala, India. Phone: 91 471 345 899. Fax: 91 471 348 096. E-mail: durg-singh{at}mailcity.com.

Journal of Clinical Microbiology, November 2002, p. 4321-4324, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4321-4324.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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