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Journal of Clinical Microbiology, November 2002, p. 4343-4345, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4343-4345.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Real-Time Fluorescence-Based PCR for Detection of Malaria Parasites

Defence Medical Research Institute, Defence Science and Technology Agency,¹ Department of Microbiology,² Department of Community and Occupational Medicine, National University of Singapore, Singapore 117597,⁴ Division of Haematology, National University Hospital, Singapore 119074³

Received 17 April 2002/ Returned for modification 7 June 2002/ Accepted 5 August 2002

A fluorescence-based real-time 5' nuclease PCR capable of detecting all four human malaria parasites was developed to screen large numbers of samples during an outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy for malaria patients can be monitored by determining the reduction of parasitemia by this method.

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