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Journal of Clinical Microbiology, November 2002, p. 4388-4390, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4388-4390.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Simple Microdilution Test for Detection of Metallo-ß-Lactamase Production in Pseudomonas aeruginosa

Roberta Migliavacca,1 Jean-Denis Docquier,2 Claudia Mugnaioli,2 Gianfranco Amicosante,3 Rossana Daturi,4 Kyungwon Lee,5 Gian Maria Rossolini,2 and Laura Pagani1*

Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sez. di Microbiologia, Università di Pavia,1 Servizio di Analisi Microbiologiche, I.R.C.C.S. S. Matteo, 27100 Pavia,4 Dipartimento di Biologia Molecolare, Sez. di Microbiologia, Università di Siena, 53100 Siena,2 Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, 67100 L'Aquila, Italy,3 Department of Clinical Pathology and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea5

Received 4 March 2002/ Returned for modification 3 April 2002/ Accepted 29 August 2002

A microdilution test measuring imipenem MICs in the presence or absence of a mixture of EDTA plus 1,10-phenanthroline was developed and tested on 190 Pseudomonas aeruginosa isolates, including 18 VIM- and 4 IMP-type metallo-ß-lactamase (MBL) producers. The chelator mixture reduced by fourfold or more the imipenem MICs for MBL producers, while a lower effect or no effect was usually observed with MBL nonproducers.

* Corresponding author. Mailing address: Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sezione di Microbiologia, Università di Pavia, Via Brambilla, 74, 27100 Pavia, Italy. Phone: 39 0382 526335. Fax: 39 0382 525886. E-mail: lpagani{at}unipv.it.

Journal of Clinical Microbiology, November 2002, p. 4388-4390, Vol. 40, No. 11
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.11.4388-4390.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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