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Journal of Clinical Microbiology, December 2002, p. 4445-4449, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4445-4449.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differences in Virulence Factors among Clinical Isolates of Escherichia coli Causing Cystitis and Pyelonephritis in Women and Prostatitis in Men

Joaquim Ruiz,1* Karine Simon,1 Juan P. Horcajada,2 Maria Velasco,2 Margarita Barranco,1 Gloria Roig,3 Antonio Moreno-Martínez,2 Jose A. Martínez,2 Teresa Jiménez de Anta,1 Josep Mensa,2 and Jordi Vila1

Servei de Microbiologia and Servei de Malalties Infeccioses,1 Institut Clínic Infecciones i Inmunología, Hospital Clínic-IDIBAPS,2 Centro de Atención Primaria Manso, Barcelona, Spain3

Received 19 August 2002/ Accepted 21 September 2002

Differences in the presence of nine urovirulence factors among clinical isolates of Escherichia coli causing cystitis and pyelonephritis in women and prostatitis in men have been studied. Hemolysin and necrotizing factor type 1 occur significantly more frequently among isolates causing prostatitis than among those causing cystitis (P < 0.0001) or pyelonephritis (P < 0.005). Moreover, the papGIII gene occurred more frequently in E. coli isolates associated with prostatitis (27%) than in those associated with pyelonephritis (9%) (P < 0.05). Genes encoding aerobactin and PapC occurred significantly less frequently in isolates causing cystitis than in those causing prostatitis (P < 0.01 and P < 0.0001, respectively) and pyelonephritis (P < 0.01 and P < 0.0001, respectively). No differences in the presence of Sat or type 1 fimbriae were found. Finally, AAFII and Bfp fimbriae are no longer considered uropathogenic virulence factors since they were not found in any of the strains analyzed. Overall, the results showed that clinical isolates producing prostatitis need greater virulence than isolates producing pyelonephritis in women or, in particular, cystitis in women (P < 0.05). Overall, the results suggest that clinical isolates producing prostatitis are more virulent that those producing pyelonephritis or cystitis in women.

* Corresponding author. Mailing address: Servicio de Microbiologia, Instituto Clínico de Infecciones e Inmunologia, IDIBAPS, Hospital Clínic, C/.Villarroel 170, 08036-Barcelona, Spain. Phone: 34932275522, ext. 2361. Fax: 34932275454. E-mail: joruiz{at}clinic.ub.es.

Journal of Clinical Microbiology, December 2002, p. 4445-4449, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4445-4449.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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