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Journal of Clinical Microbiology, December 2002, p. 4486-4492, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4486-4492.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Diversity of Intimin Genes of Attaching and Effacing Escherichia coli Strains

W. L. Zhang,1 B. Köhler,2 E. Oswald,3 L. Beutin,4 H. Karch,1 S. Morabito,5 A. Caprioli,5 S. Suerbaum,2 and H. Schmidt2*

Institut für Hygiene der Westfälischen Wilhelms-Universität, 48149 Münster,1 Institut für Hygiene und Mikrobiologie der Bayerischen Julius-Maximilians-Universität, 97080 Würzburg,2 Division of Emerging Bacterial Pathogens, Robert Koch Institut, 13353 Berlin, Germany,4 UMR960 INRA de Microbiologie Moleculaire, Ecole Nationale Veterinaire, 31000 Toulouse, France,3 Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanita, 00161-Rome, Italy5

Received 21 June 2002/ Returned for modification 5 August 2002/ Accepted 12 September 2002

In this study, we determined the sequences of four intimin variant genes detected in attaching and effacing Escherichia coli isolates of human origin. Three of them were novel and were designated eae-{eta} (eta), eae-{iota} (iota), and eae-{kappa} (kappa). The fourth was identical to the recently described eae-{zeta} (zeta), isolated from a bovine E. coli O84:NM isolate. We compared these sequences with those of published intimin-{alpha}, intimin-ß, intimin-{gamma}1, intimin-{gamma}2, intimin-{varepsilon}, and intimin-{theta} alleles. Sequence analysis of these 10 intimin alleles confirmed extensive genetic diversity within the intimin gene family in E. coli. The genetic diversity was more prominent in the 3' region (starting at bp 2112), which encodes the binding domain of intimin. Phylogenetic analyses revealed four groups of closely related intimin genes: {alpha} and {zeta}; ß and {kappa}; {gamma}1 and {gamma}2/{theta}; and {varepsilon} and {eta}. Calculation of homoplasy ratios of sequences of the 5' region of eae (positions 1 to 2111) revealed evidence for intragenic recombination. Split decomposition analysis also indicates that recombination events have played a role in the evolutionary history of eae. In conclusion, we recommend an eae nomenclature system based on the Greek alphabet and provide an updated PCR scheme for amplification and typing of E. coli eae.

* Corresponding author. Present address: Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, Germany. Phone: 49-351/458-6570. Fax: 49-351/458-6310. E-mail: Herbert.Schmidt{at}mailbox.tu-dresden.de.

Journal of Clinical Microbiology, December 2002, p. 4486-4492, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4486-4492.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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