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Journal of Clinical Microbiology, December 2002, p. 4544-4546, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4544-4546.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Determining the Genetic Structure of the Natural Population of Staphylococcus aureus: a Comparison of Multilocus Sequence Typing with Pulsed-Field Gel Electrophoresis, Randomly Amplified Polymorphic DNA Analysis, and Phage Typing

Hajo Grundmann,1* Satoshi Hori,1 Mark C. Enright,2 Carol Webster,1 Adriana Tami,1 Edward J. Feil,2 and Tyrone Pitt3

Division of Microbiology and Infectious Diseases, University Hospital Nottingham, Nottingham NG7 2UH,1 Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY,2 Laboratory of Hospital Infection, Division of Nosocomial Infection Prevention and Control, Central Public Health Laboratory, London NW9 5HT, United Kingdom3

Received 15 April 2002/ Returned for modification 3 August 2002/ Accepted 25 September 2002

We used a sample of Staphylococcus aureus strains that are carried by humans and that are representative of the natural population of S. aureus strains in order to assess the value of multilocus sequence typing (MLST), pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing as epidemiological tools. Only MLST was able to define clonal complexes unambiguously. All DNA-based typing approaches achieved a high degree of agreement, implying phylogenetic concordance, but predicted epidemiological associations with variable accuracy.

* Corresponding author. Mailing address: Division of Microbiology and Infectious Diseases, Queen's Medical Centre, University Hospital Nottingham, Nottingham NG7 2UH, United Kingdom. Phone: 44-115-970 9162. Fax: 44-115-970 9233. E-mail: Hajo.Grundmann{at}Nottingham.ac.uk.

Journal of Clinical Microbiology, December 2002, p. 4544-4546, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4544-4546.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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