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Journal of Clinical Microbiology, December 2002, p. 4760-4762, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4760-4762.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mycobacterium avium subsp. paratuberculosis Strains from Cattle and Sheep Can Be Distinguished by a PCR Test Based on a Novel DNA Sequence Difference

Desmond M. Collins,* May De Zoete, and Sonia M. Cavaignac

AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand

Received 10 June 2002/ Returned for modification 2 September 2002/ Accepted 22 September 2002

A DNA sequence differing between sheep and cattle types of Mycobacterium avium subsp. paratuberculosis was identified and used to develop a PCR test. The test unequivocally distinguished all sheep types from cattle types and was negative for a wide range of other strains from the Mycobacterium avium-Mycobacterium intracellulare complex. The test will be useful for epidemiological purposes, particularly in hosts such as deer that can be easily infected with either type.

* Corresponding author. Mailing address: AgResearch, Wallaceville Animal Research Centre, P.O. Box 40063, Upper Hutt, New Zealand. Phone: 64-4-922-1310. Fax: 64-4-922-1413. E-mail: desmond.collins{at}agresearch.co.nz.

Journal of Clinical Microbiology, December 2002, p. 4760-4762, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4760-4762.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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