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Journal of Clinical Microbiology, December 2002, p. 4765, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4765-4767.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Overestimation of the Hepatitis C Virus RNA Content of Reference Preparations by the AMPLICOR HCV Monitor Test, Version 2.0

Giulio Pisani, Karen Cristiano, Maria Wirz, Guillermo M. Bisso, and Giuliano Gentili*

Laboratory of Immunology, Istituto Superiore di Sanità, Rome, Italy

Received 19 April 2002/ Returned for modification 16 June 2002/ Accepted 25 September 2002

An evaluation of the AMPLICOR hepatitis C virus (HCV) monitor test, version 2.0 (Roche Diagnostics), was carried out to investigate whether this test overestimates the HCV RNA content of reference preparations. Satisfactory accuracy was observed when the World Health Organization HCV international standard was included in the assay and a modified formula was used to calculate the viral content.

* Corresponding author. Mailing address: Laboratory of Immunology, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy. Phone: 39-06-49902346. Fax: 39-06-49387115. E-mail: ggentili{at}iss.it.

Journal of Clinical Microbiology, December 2002, p. 4765, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4765-4767.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology. All rights reserved.