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Journal of Clinical Microbiology, December 2002, p. 4768-4770, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4768-4770.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Persistence of Pigment Production by Yeast Isolates Grown on CHROMagar Candida Medium

Duane R. Hospenthal,1* Clinton K. Murray,1 Miriam L. Beckius,2 Judith A. Green,2 and David P. Dooley1

Department of Medicine,1 Department of Pathology, Brooke Army Medical Center, Fort Sam Houston, Texas2

Received 22 March 2002/ Returned for modification 15 July 2002/ Accepted 25 September 2002

We evaluated the persistence of pigmentation in yeast isolates grown on the chromogenic medium CHROMagar Candida over 7 days. Candida, Cryptococcus, and Trichosporon isolates were inoculated alone or mixed onto duplicate sets of plates and incubated at 30 and 35°C. Candida albicans and Candida krusei were readily identified throughout the reading period, but Candida glabrata was difficult to differentiate from other species until the 3- or 4-day time point. Candida tropicalis produced colonies similar to those of rare Cryptococcus and Trichosporon species, and mixed cultures were often difficult to identify as such.

* Corresponding author. Mailing address: Brooke Army Medical Center, Infectious Disease (MCHE-MDI), 3851 Roger Brooke Dr., Fort Sam Houston, TX 78234. Phone: (210) 916-4355. Fax: (210) 916-0388. E-mail: Duane.Hospenthal{at}amedd.army.mil.

Journal of Clinical Microbiology, December 2002, p. 4768-4770, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4768-4770.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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