This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Limansky, A. S. Articles by Viale, A. M. Search for Related Content PubMed PubMed Citation Articles by Limansky, A. S. Articles by Viale, A. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2002, p. 4776-4778, Vol. 40, No. 12
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.12.4776-4778.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Loss of a 29-Kilodalton Outer Membrane Protein in Acinetobacter baumannii Is Associated with Imipenem Resistance

Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET) and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000 Rosario, Argentina

Received 14 June 2002/ Returned for modification 16 August 2002/ Accepted 23 September 2002

We analyzed the possible causes of imipenem (IPM) resistance in multidrug-resistant isolates of Acinetobacter baumannii. Comparison of the outer membrane protein (OMP) profiles of two genomically related strains (Ab288 [IPM sensitive] and Ab242 [IPM resistant]) indicated the conspicuous loss of a 29-kDa polypeptide in the Ab242 strain. No carbapenemase activity was detected in any of these strains. The treatment of Ab288 with sodium salicylate resulted in IPM resistance and the loss of the 29-kDa OMP. In addition, IPM-resistant clones of Ab288 which were selected by repetitive culturing in increasing concentrations of this antibiotic also showed the absence of this 29-kDa OMP.

This article has been cited by other articles:

• Bratu, S., Landman, D., Martin, D. A., Georgescu, C., Quale, J. (2008). Correlation of Antimicrobial Resistance with {beta}-Lactamases, the OmpA-Like Porin, and Efflux Pumps in Clinical Isolates of Acinetobacter baumannii Endemic to New York City. Antimicrob. Agents Chemother. 52: 2999-3005 [Abstract] [Full Text]  
• Peleg, A. Y., Seifert, H., Paterson, D. L. (2008). Acinetobacter baumannii: Emergence of a Successful Pathogen. Clin. Microbiol. Rev. 21: 538-582 [Abstract] [Full Text]  
• Chen, T.-L., Wu, R. C.-C., Shaio, M.-F., Fung, C.-P., Cho, W.-L. (2008). Acquisition of a Plasmid-Borne blaOXA-58 Gene with an Upstream IS1008 Insertion Conferring a High Level of Carbapenem Resistance to Acinetobacter baumannii. Antimicrob. Agents Chemother. 52: 2573-2580 [Abstract] [Full Text]  
• Beceiro, A., Fernandez-Cuenca, F., Ribera, A., Martinez-Martinez, L., Pascual, A., Vila, J., Rodriguez-Bano, J., Cisneros, J. M., Pachon, J., Bou, G., on behalf of the Spanish Group for Nosocomial Infe, (2008). False extended-spectrum {beta}-lactamase detection in Acinetobacter spp. due to intrinsic susceptibility to clavulanic acid. J Antimicrob Chemother 61: 301-308 [Abstract] [Full Text]  
• Hu, W. S., Yao, S.-M., Fung, C.-P., Hsieh, Y.-P., Liu, C.-P., Lin, J.-F. (2007). An OXA-66/OXA-51-Like Carbapenemase and Possibly an Efflux Pump Are Associated with Resistance to Imipenem in Acinetobacter baumannii. Antimicrob. Agents Chemother. 51: 3844-3852 [Abstract] [Full Text]  
• Perez, F., Hujer, A. M., Hujer, K. M., Decker, B. K., Rather, P. N., Bonomo, R. A. (2007). Global Challenge of Multidrug-Resistant Acinetobacter baumannii. Antimicrob. Agents Chemother. 51: 3471-3484 [Full Text]  
• Cerquetti, M., Giufre, M., Cardines, R., Mastrantonio, P. (2007). First Characterization of Heterogeneous Resistance to Imipenem in Invasive Nontypeable Haemophilus influenzae Isolates. Antimicrob. Agents Chemother. 51: 3155-3161 [Abstract] [Full Text]  
• Valenzuela, J. K., Thomas, L., Partridge, S. R., van der Reijden, T., Dijkshoorn, L., Iredell, J. (2007). Horizontal Gene Transfer in a Polyclonal Outbreak of Carbapenem-Resistant Acinetobacter baumannii. J. Clin. Microbiol. 45: 453-460 [Abstract] [Full Text]  
• Depardieu, F., Podglajen, I., Leclercq, R., Collatz, E., Courvalin, P. (2007). Modes and Modulations of Antibiotic Resistance Gene Expression. Clin. Microbiol. Rev. 20: 79-114 [Abstract] [Full Text]  
• Walther-Rasmussen, J., Hoiby, N. (2006). OXA-type carbapenemases. J Antimicrob Chemother 57: 373-383 [Abstract] [Full Text]  
• Siroy, A., Molle, V., Lemaitre-Guillier, C., Vallenet, D., Pestel-Caron, M., Cozzone, A. J., Jouenne, T., De, E. (2005). Channel Formation by CarO, the Carbapenem Resistance-Associated Outer Membrane Protein of Acinetobacter baumannii. Antimicrob. Agents Chemother. 49: 4876-4883 [Abstract] [Full Text]  
• Tomas, M. d. M., Beceiro, A., Perez, A., Velasco, D., Moure, R., Villanueva, R., Martinez-Beltran, J., Bou, G. (2005). Cloning and Functional Analysis of the Gene Encoding the 33- to 36-Kilodalton Outer Membrane Protein Associated with Carbapenem Resistance in Acinetobacter baumannii. Antimicrob. Agents Chemother. 49: 5172-5175 [Abstract] [Full Text]  
• Su, X.-Z., Chen, J., Mizushima, T., Kuroda, T., Tsuchiya, T. (2005). AbeM, an H+-Coupled Acinetobacter baumannii Multidrug Efflux Pump Belonging to the MATE Family of Transporters. Antimicrob. Agents Chemother. 49: 4362-4364 [Abstract] [Full Text]  
• Mussi, M. A., Limansky, A. S., Viale, A. M. (2005). Acquisition of Resistance to Carbapenems in Multidrug-Resistant Clinical Strains of Acinetobacter baumannii: Natural Insertional Inactivation of a Gene Encoding a Member of a Novel Family of {beta}-Barrel Outer Membrane Proteins. Antimicrob. Agents Chemother. 49: 1432-1440 [Abstract] [Full Text]  
• Nagano, N., Nagano, Y., Cordevant, C., Shibata, N., Arakawa, Y. (2004). Nosocomial Transmission of CTX-M-2 {beta}-Lactamase-Producing Acinetobacter baumannii in a Neurosurgery Ward. J. Clin. Microbiol. 42: 3978-3984 [Abstract] [Full Text]