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Journal of Clinical Microbiology, February 2002, p. 325-329, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.02.325-329.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Highly Sensitive Assay for Human Herpesvirus 8 Antibodies That Uses a Multiple Antigenic Peptide Derived from Open Reading Frame K8.1

Lee L. Lam,1* Chou-Pong Pau,1 Sheila C. Dollard,2 Philip E. Pellett,2 and Thomas J. Spira1

Division of AIDS, STD, and TB Laboratory Research,1 Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332

Received 9 March 2001/ Returned for modification 10 July 2001/ Accepted 14 August 2001

The immunodominant region of the human herpesvirus 8 (HHV-8), the antibody-binding site of glycoprotein K8.1A, was mapped to the N-terminal region by using overlapping peptides and a residue replacement method. The main epitope was located within residues 44 to 56 (GQVYQDWL----C). Based on this information, we developed an enzyme immunoassay to detect HHV-8 antibodies in human sera using a four-branch multiple antigenic peptide as the antigen. The sensitivity and specificity of the assay were 96 and 99.4%, respectively. This assay should be useful for population-based, epidemiological studies of HHV-8 infection.

* Corresponding author. Mailing address: Division of AIDS, STD, and Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mail Stop A25, Atlanta, GA 30333. Phone: (404) 639-2725. Fax: (404) 639-2660. E-mail: lxl7{at}cdc.gov.

Journal of Clinical Microbiology, February 2002, p. 325-329, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.02.325-329.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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