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Journal of Clinical Microbiology, February 2002, p. 325-329, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.02.325-329.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Highly Sensitive Assay for Human Herpesvirus 8 Antibodies That Uses a Multiple Antigenic Peptide Derived from Open Reading Frame K8.1

Division of AIDS, STD, and TB Laboratory Research,¹ Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333²

Received 9 March 2001/ Returned for modification 10 July 2001/ Accepted 14 August 2001

The immunodominant region of the human herpesvirus 8 (HHV-8), the antibody-binding site of glycoprotein K8.1A, was mapped to the N-terminal region by using overlapping peptides and a residue replacement method. The main epitope was located within residues 44 to 56 (GQVYQDWL----C). Based on this information, we developed an enzyme immunoassay to detect HHV-8 antibodies in human sera using a four-branch multiple antigenic peptide as the antigen. The sensitivity and specificity of the assay were 96 and 99.4%, respectively. This assay should be useful for population-based, epidemiological studies of HHV-8 infection.

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