This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Atanassov, C. Articles by Fauchère, J.-L. Search for Related Content PubMed PubMed Citation Articles by Atanassov, C. Articles by Fauchère, J.-L.

Novel Antigens of Helicobacter pylori Correspond to Ulcer-Related Antibody Pattern of Sera from Infected Patients

Department of Microbiology A, IFR 59, University Hospital Center, 86021 Poitiers,¹ Laboratory of Protein Microsequencing, Institut Pasteur, 75724 Paris,² Laboratory of Microbiology, Faculty of Medicine, 29200 Brest, France³

Received 15 June 2001/ Returned for modification 24 September 2001/ Accepted 18 November 2001

Recently, we reported that the patterns of antibodies to Helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers (P. Aucher et al., J. Clin. Microbiol. 36:931-936, 1998). Here we report the identification, by a combination of electrophoretic, immunochemical, and protein sequencing methods, of five antigens that correspond to this antibody pattern: groEL, catalase A, flagellin A, beta-ketoacyl-acyl carrier protein synthase I (ß-ketoacyl-ACP S), and peptidyl prolyl cis-trans isomerase (PPiase). ß-Ketoacyl-ACP S and PPiase are reported for the first time as antigens of diagnostic interest in infections by H. pylori. The antigenicity of the five antigens, together with those of CagA and VacA, was tested in an immunoblot assay with water-soluble protein extracts from two H. pylori pathogenic strains (HP 141 and ATCC 43579) and panels of sera from H. pylori-positive patients with gastroduodenal ulcers (GDU), nonulcer dyspepsia (NUD), as well as sera from H. pylori-negative healthy volunteers. For catalase A, groEL, and flagellin A antigens, no overall statistically important values were found making it possible to discriminate between patients with GDU and NUD. For both H. pylori strains, the mean performance indices (MPI) presenting percentages of correctly classified patients with GDU and NUD showed that the most significant antibody patterns were as follows: anti-VacA + anti-ß-ketoacyl-ACP S (MPI = 76.1), anti-VacA + anti-PPiase (MPI = 71.8), and anti-CagA + anti-VacA + anti-ß-ketoacyl-ACP S (MPI = 70.5). Antibody patterns detected with these antigen profiles may therefore be useful in developing a diagnostic test designed to predict the clinical severity of the H. pylori infection within the adult population of France.

This article has been cited by other articles:

• Lin, Y.-F., Chen, C.-Y., Tsai, M.-H., Wu, M.-S., Wang, Y.-C., Chuang, E. Y., Lin, J.-T., Yang, P.-C., Chow, L.-P. (2007). Duodenal Ulcer-related Antigens from Helicobacter pylori: Immunoproteome and Protein Microarray Approaches. Mol. Cell. Proteomics 6: 1018-1026 [Abstract] [Full Text]  
• Pathak, S. K., Basu, S., Bhattacharyya, A., Pathak, S., Banerjee, A., Basu, J., Kundu, M. (2006). TLR4-Dependent NF-{kappa}B Activation and Mitogen- and Stress-Activated Protein Kinase 1-Triggered Phosphorylation Events Are Central to Helicobacter pylori Peptidyl Prolyl cis-, trans-Isomerase (HP0175)-Mediated Induction of IL-6 Release from Macrophages. J. Immunol. 177: 7950-7958 [Abstract] [Full Text]  
• Henderson, B., Allan, E., Coates, A. R. M. (2006). Stress Wars: the Direct Role of Host and Bacterial Molecular Chaperones in Bacterial Infection. Infect. Immun. 74: 3693-3706 [Full Text]  
• Basak, C., Pathak, S. K., Bhattacharyya, A., Pathak, S., Basu, J., Kundu, M. (2005). The Secreted Peptidyl Prolyl cis,trans-Isomerase HP0175 of Helicobacter pylori Induces Apoptosis of Gastric Epithelial Cells in a TLR4- and Apoptosis Signal-Regulating Kinase 1-Dependent Manner. J. Immunol. 174: 5672-5680 [Abstract] [Full Text]  
• Kim, N., Weeks, D. L., Shin, J. M., Scott, D. R., Young, M. K., Sachs, G. (2002). Proteins Released by Helicobacter pylori In Vitro. J. Bacteriol. 184: 6155-6162 [Abstract] [Full Text]