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Journal of Clinical Microbiology, February 2002, p. 611-614, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.611-614.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Extended-Spectrum ß-Lactamases Produced by Nosocomial Isolates of Enterobacteriaceae from an Italian Nationwide Survey

Mariagrazia Perilli,1 Emanuela Dell'Amico,2 Bernardetta Segatore,1 Maria Rosaria de Massis,1 Ciro Bianchi,3 Francesco Luzzaro,4 Gian Maria Rossolini,2 Antonio Toniolo,4 Giuseppe Nicoletti,5 and Gianfranco Amicosante1,6*

Dipartimento di Scienze e Tecnologie Biomediche,1 Cattedra di Biochimica Clinica, Università di L'Aquila, L'Aquila,6 Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Siena,2 Dipartimento MedicoWyeth-Lederle Italia S.p.A., Aprilia,3 Ospedale di Circolo di Varese, Università dell' Insubria, Varese,4 Università di Catania, Catania, Italy5

Received 31 July 2001/ Returned for modification 3 October 2001/ Accepted 2 December 2001

Extended-spectrum ß-lactamases (ESBLs) are widespread in hospital settings worldwide. The present investigation was undertaken to assess the distribution and prevalence of ESBLs belonging to the TEM and SHV families in 448 ESBL-producing clinical isolates of Enterobacteriaceae collected from 10 different Italian hospitals. The natures of TEM and SHV determinants were identified by direct sequencing of PCR-amplified genes. TEM-52 and SHV-12 were the most common variants, and they were found in most hospitals and in several different species. Other less frequent variants included TEM-5, TEM-12, TEM-15, TEM-19, TEM-20, TEM-24, TEM-26, TEM-43, TEM-60, TEM-72, TEM-87, SHV-2a, SHV-5, and SHV-11. Proteus mirabilis was the most common producer of TEM-type ESBLs, while Klebsiella pneumoniae was the most common producer of SHV-type ESBLs. The distribution of TEM- and SHV-type ESBL variants in Enterobacteriaceae from Italian hospitals exhibited notable differences from those from other geographical settings.

* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Biomediche, Cattedra di Biochimica Clinica, Università di L'Aquila, Località Coppito, Via Vetoio, 67010 L'Aquila, Italy. Phone: 39 0862 433455. Fax: 39 0862 433433. E-mail: amicosante{at}cc.univaq.it.

Journal of Clinical Microbiology, February 2002, p. 611-614, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.611-614.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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