This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hirose, K. Articles by Watanabe, H. Search for Related Content PubMed PubMed Citation Articles by Hirose, K. Articles by Watanabe, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2002, p. 633-636, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.02.633-636.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Selective Amplification of tyv (rfbE), prt (rfbS), viaB, and fliC Genes by Multiplex PCR for Identification of Salmonella enterica Serovars Typhi and Paratyphi A

Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640,¹ Department of Microbiology, National Institute of Public Health, Tokyo 108-8638,² Department of Microbiology, Okayama Prefectural Institute for Environmental Science and Public Health, Okayama 701-0298,³ Saitama Institute for Public Health, Saitama 338-0842,⁴ Saga Prefectural Institute of Public Health, Saga 849-0925,⁵ Department of Microbiology, Gifu University, School of Medicine, Gifu 500-8750, Japan⁶

Received 28 June 2001/ Returned for modification 26 July 2001/ Accepted 26 November 2001

The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS), fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.

This article has been cited by other articles:

• Levy, H., Diallo, S., Tennant, S. M., Livio, S., Sow, S. O., Tapia, M., Fields, P. I., Mikoleit, M., Tamboura, B., Kotloff, K. L., Lagos, R., Nataro, J. P., Galen, J. E., Levine, M. M. (2008). PCR Method To Identify Salmonella enterica Serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the Blood of Patients with Clinical Enteric Fever. J. Clin. Microbiol. 46: 1861-1866 [Abstract] [Full Text]  
• Saroj, S. D., Shashidhar, R., Karani, M., Bandekar, J. R. (2008). Distribution of Salmonella pathogenicity island (SPI)-8 and SPI-10 among different serotypes of Salmonella. J Med Microbiol 57: 424-427 [Abstract] [Full Text]  
• Ou, H.-Y., Ju, C. T. S., Thong, K.-L., Ahmad, N., Deng, Z., Barer, M. R., Rajakumar, K. (2007). Translational Genomics to Develop a Salmonella enterica Serovar Paratyphi A Multiplex Polymerase Chain Reaction Assay. J. Mol. Diagn. 9: 624-630 [Abstract] [Full Text]  
• Kim, H.-J., Park, S.-H., Kim, H.-Y. (2006). Comparison of Salmonella enterica Serovar Typhimurium LT2 and Non-LT2 Salmonella Genomic Sequences, and Genotyping of Salmonellae by Using PCR. Appl. Environ. Microbiol. 72: 6142-6151 [Abstract] [Full Text]  
• Tracz, D. M., Tabor, H., Jerome, M., Ng, L.-K., Gilmour, M. W. (2006). Genetic Determinants and Polymorphisms Specific for Human-Adapted Serovars of Salmonella enterica That Cause Enteric Fever.. J. Clin. Microbiol. 44: 2007-2018 [Abstract] [Full Text]  
• Baker, S., Sarwar, Y., Aziz, H., Haque, A., Ali, A., Dougan, G., Wain, J., Haque, A. (2005). Detection of Vi-Negative Salmonella enterica Serovar Typhi in the Peripheral Blood of Patients with Typhoid Fever in the Faisalabad Region of Pakistan. J. Clin. Microbiol. 43: 4418-4425 [Abstract] [Full Text]  
• Morin, N. J., Gong, Z., Li, X.-F. (2004). Reverse Transcription-Multiplex PCR Assay for Simultaneous Detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi. Clin. Chem. 50: 2037-2044 [Abstract] [Full Text]  
• Koropatkin, N. M., Liu, H.-w., Holden, H. M. (2003). High Resolution X-ray Structure of Tyvelose Epimerase from Salmonella typhi. J. Biol. Chem. 278: 20874-20881 [Abstract] [Full Text]