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Journal of Clinical Microbiology, February 2002, p. 645-648, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.645-648.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Diffusely Adherent Escherichia coli as a Cause of Acute Diarrhea in Young Children in Northeast Brazil: a Case-Control Study

Isabel C. A. Scaletsky,1* Sandra H. Fabbricotti,1 Rozane L. B. Carvalho,2 Claudia R. Nunes,3 Helcio S. Maranhão,4 Mauro B. Morais,2 and Ulysses Fagundes-Neto2

Departamento de Microbiologia, Imunologia e Parasitologia,1 Disciplina de Gastroenterologia Pediátrica,2 Universidade Federal de São Paulo, São Paulo, SP, Hospital Universitário Materno-Infantil, São Luis, MA,3 Departamento de Pediatria, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil4

Received 16 July 2001/ Returned for modification 17 September 2001/ Accepted 9 November 2001

In a prospective study carried out in two urban centers in northeastern Brazil, 195 HEp-2-adherent Escherichia coli strains were isolated; 110 were identified as the only pathogen in stools of children with diarrhea, and 85 were from controls. Enteropathogenic E. coli isolates were identified in 21 children with diarrhea (8.9%) and 7 children without diarrhea (3.0%), and they were significantly associated with diarrhea (P < 0.01). Enteroaggregative E. coli strains were isolated from 40 children with diarrhea (16.9%) and 38 children without diarrhea (16.4%) and showed no correlation with diarrhea (P > 0.5). In 49 children with diarrhea (20.7%) and 40 children without diarrhea (17.3%), diffusely adherent E. coli (DAEC) isolates were detected and were not found to be associated with diarrhea (P = 0.41). However, after stratification, for children older than 12 months of age a significant correlation between DAEC infection and diarrhea was detected (P = 0.01). These results suggest that DAEC isolates should be considered potential pathogens in northeastern Brazil and also confirm the association of DAEC with age-dependent diarrhea.

* Corresponding author. Mailing address: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Sãu Paulo, Brazil. Phone: 55-11-55764537. Fax: 55-11-55716504. E-mail: scaletsky{at}ecb.epm.br.

Journal of Clinical Microbiology, February 2002, p. 645-648, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.645-648.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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