This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pettigrew, M. M. Articles by Gilsdorf, J. Search for Related Content PubMed PubMed Citation Articles by Pettigrew, M. M. Articles by Gilsdorf, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2002, p. 660-662, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.660-662.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of Pulsed-Field Gel Electrophoresis, Enterobacterial Repetitive Intergenic Consensus Typing, and Automated Ribotyping To Assess Genomic Variability among Strains of Nontypeable Haemophilus influenzae

Department of Epidemiology, University of Michigan, Ann Arbor, Michigan 48109-2029,,¹ Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109-0244²

Received 6 August 2001/ Returned for modification 27 September 2001/ Accepted 1 November 2001

We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques as the sole method to group NT H. influenzae strains.

This article has been cited by other articles:

• Berrens, Z. J., Marrs, C. F., Pettigrew, M. M., Sandstedt, S. A., Patel, M., Gilsdorf, J. R. (2007). Genetic Diversity of Paired Middle-Ear and Pharyngeal Nontypeable Haemophilus influenzae Isolates from Children with Acute Otitis Media. J. Clin. Microbiol. 45: 3764-3767 [Abstract] [Full Text]  
• Mukundan, D., Ecevit, Z., Patel, M., Marrs, C. F., Gilsdorf, J. R. (2007). Pharyngeal Colonization Dynamics of Haemophilus influenzae and Haemophilus haemolyticus in Healthy Adult Carriers. J. Clin. Microbiol. 45: 3207-3217 [Abstract] [Full Text]  
• Kim, I.-S., Ki, C.-S., Kim, S., Oh, W. S., Peck, K. R., Song, J.-H., Lee, K., Lee, N. Y. (2007). Diversity of Ampicillin Resistance Genes and Antimicrobial Susceptibility Patterns in Haemophilus influenzae Strains Isolated in Korea. Antimicrob. Agents Chemother. 51: 453-460 [Abstract] [Full Text]  
• Srinivasan, U., Zhang, L., France, A. M., Ghosh, D., Shalaby, W., Xie, J., Marrs, C. F., Foxman, B. (2007). Probe Hybridization Array Typing: a Binary Typing Method for Escherichia coli. J. Clin. Microbiol. 45: 206-214 [Abstract] [Full Text]  
• Giufre, M., Muscillo, M., Spigaglia, P., Cardines, R., Mastrantonio, P., Cerquetti, M. (2006). Conservation and Diversity of HMW1 and HMW2 Adhesin Binding Domains among Invasive Nontypeable Haemophilus influenzae Isolates. Infect. Immun. 74: 1161-1170 [Abstract] [Full Text]  
• Mbwana, J., Bolin, I., Lyamuya, E., Mhalu, F., Lagergard, T. (2006). Molecular Characterization of Haemophilus ducreyi Isolates from Different Geographical Locations. J. Clin. Microbiol. 44: 132-137 [Abstract] [Full Text]  
• Watson, M. E. Jr, Burns, J. L., Smith, A. L. (2004). Hypermutable Haemophilus influenzae with mutations in mutS are found in cystic fibrosis sputum. Microbiology 150: 2947-2958 [Abstract] [Full Text]  
• Hussain, M. A., Kauser, F., Khan, A. A., Tiwari, S., Habibullah, C. M., Ahmed, N. (2004). Implications of Molecular Genotyping of Helicobacter pylori Isolates from Different Human Populations by Genomic Fingerprinting of Enterobacterial Repetitive Intergenic Consensus Regions for Strain Identification and Geographic Evolution. J. Clin. Microbiol. 42: 2372-2378 [Abstract] [Full Text]  
• Kaczmarek, F. S., Gootz, T. D., Dib-Hajj, F., Shang, W., Hallowell, S., Cronan, M. (2004). Genetic and Molecular Characterization of {beta}-Lactamase-Negative Ampicillin-Resistant Haemophilus influenzae with Unusually High Resistance to Ampicillin. Antimicrob. Agents Chemother. 48: 1630-1639 [Abstract] [Full Text]  
• Gilsdorf, J. R., Marrs, C. F., Foxman, B. (2004). Haemophilus influenzae: Genetic Variability and Natural Selection To Identify Virulence Factors. Infect. Immun. 72: 2457-2461 [Full Text]  
• Munson, R. S. Jr., Harrison, A., Gillaspy, A., Ray, W. C., Carson, M., Armbruster, D., Gipson, J., Gipson, M., Johnson, L., Lewis, L., Dyer, D. W., Bakaletz, L. O. (2004). Partial Analysis of the Genomes of Two Nontypeable Haemophilus influenzae Otitis Media Isolates. Infect. Immun. 72: 3002-3010 [Abstract] [Full Text]  
• Meacham, K. J., Zhang, L., Foxman, B., Bauer, R. J., Marrs, C. F. (2003). Evaluation of Genotyping Large Numbers of Escherichia coli Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR. J. Clin. Microbiol. 41: 5224-5226 [Abstract] [Full Text]  
• Augustynowicz, E., Gzyl, A., Szenborn, L., Banys, D., Gniadek, G., Slusarczyk, J. (2003). Comparison of usefulness of randomly amplified polymorphic DNA and amplified-fragment length polymorphism techniques in epidemiological studies on nasopharyngeal carriage of non-typable Haemophilus influenzae. J Med Microbiol 52: 1005-1014 [Abstract] [Full Text]  
• Bruant, G., Watt, S., Quentin, R., Rosenau, A. (2003). Typing of Nonencapsulated Haemophilus Strains by Repetitive-Element Sequence-Based PCR Using Intergenic Dyad Sequences. J. Clin. Microbiol. 41: 3473-3480 [Abstract] [Full Text]