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Quantification of Human Immunodeficiency Virus Type 1 Proviral DNA by Using TaqMan Technology

Children's Memorial Institute for Education and Research,¹ Center for Biotechnology, Northwestern University,² Departments of Pediatrics,,³ Microbiology-Immunology ,⁴ Neurosurgery Northwestern University Medical School, Chicago, Illinois⁵

Received 14 May 2001/ Returned for modification 20 August 2001/ Accepted 24 November 2001

A protocol for quantification of human immunodeficiency virus type 1 (HIV-1) proviral DNA with the TaqMan technology was developed and validated. The assay was specific for HIV-1, with an analytic sensitivity of 10 copies and a linear dynamic range of >6 logs. Viral RNA levels, when at a stable state, were highly correlated with proviral DNA levels in 80 specimens of 18 HIV-infected children.

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