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Journal of Clinical Microbiology, February 2002, p. 679-681, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.679-681.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PCR M Typing: a New Method for Rapid Typing of Group A Streptococci

Luca A. Vitali, Claudia Zampaloni, Manuela Prenna, and Sandro Ripa*

Department of Molecular Cellular Animal Biology, Chair of Microbiology, University of Camerino, 62032 Camerino, Italy

Received 9 July 2001/ Returned for modification 29 August 2001/ Accepted 27 November 2001

A new approach for the M-typing of Streptococcus pyogenes is reported. Oligonucleotide primers were used in a PCR to amplify the N-terminal region of the emm gene. The presence of the PCR amplification product is associated with the corresponding M serotype. This technique offers potential advantages over other molecular typing methods.

* Corresponding author. Mailing address: Department of Biology MCA, Chair of Microbiology, University of Camerino, 62032 Camerino (MC), Italy. Phone: 39-0737-403241. Fax: 39-0737-636216. E-mail: ripa{at}cambio.unicam.it.

Journal of Clinical Microbiology, February 2002, p. 679-681, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.679-681.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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