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Journal of Clinical Microbiology, February 2002, p. 682-684, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.682-684.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Real-Time PCR for Determination of Penicillin Susceptibility in Pneumococcal Meningitis, Including Culture-Negative Cases

Angela M. Kearns,1* Clive Graham,2 Doreen Burdess,1 Jane Heatherington,1 and Roger Freeman1

Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE,1 Department of Microbiology, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, United Kingdom2

Received 13 August 2001/ Returned for modification 13 September 2001/ Accepted 9 November 2001

A novel real-time PCR-hybridization assay was developed for the rapid (<1 h) detection of penicillin susceptibility in Streptococcus pneumoniae. When applied to 24 pneumococcal DNA-positive cerebrospinal fluid extracts, penicillin-sensitive S. pneumoniae was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases.

* Corresponding author. Mailing address: Public Health Laboratory, Newcastle General Hospital, Westgate Rd., Newcastle upon Tyne NE4 6BE, United Kingdom. Phone: 44 191 2261074. Fax: 44 191 2260365. E-mail: newakear{at}north.phls.nhs.uk.

Journal of Clinical Microbiology, February 2002, p. 682-684, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.682-684.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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