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Journal of Clinical Microbiology, February 2002, p. 690-693, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.690-693.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of Neorickettsia risticii in Adult Aquatic Insects in Pennsylvania, in Horses Infected by Ingestion of Insects, and Isolated in Cell Culture

Jason Mott,1 Yasukazu Muramatsu,1 Elizabeth Seaton,1 Carol Martin,2 Stephen Reed,3 and Yasuko Rikihisa1*

Department of Veterinary Biosciences,1 Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-1092,3 Sunbury, Pennsylvania2

Received 7 June 2001/ Returned for modification 24 September 2001/ Accepted 3 December 2001

Upon ingestion of adult aquatic insects, horses developed clinical signs of Potomac horse fever, and Neorickettsia risticii was isolated from the blood. 16S rRNA and 51-kDa antigen gene sequences from blood, isolates, and caddis flies fed to the horses were identical, proving oral transmission of N. risticii from caddis flies to horses.


* Corresponding author. Mailing address: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd., Columbus, OH 43210-1092. Phone: (614) 292-5661. Fax. (614) 292-6473. E-mail: Rikihisa.1{at}osu.edu.


Journal of Clinical Microbiology, February 2002, p. 690-693, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.690-693.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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Copyright © 2002 by the American Society for Microbiology. All rights reserved.