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Journal of Clinical Microbiology, February 2002, p. 698-703, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.698-703.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Specific Identification of Listeria welshimeri and Listeria monocytogenes by PCR Assays Targeting a Gene Encoding a Fibronectin-Binding Protein

Philippe Gilot* and Jean Content

Department of Virology, Pasteur Institute, B-1180 Brussels, Belgium

Received 27 August 2001/ Returned for modification 14 November 2001/ Accepted 26 November 2001

We have cloned and sequenced a Listeria welshimeri DNA fragment homologous to the previously described fibronectin-binding protein-encoding gene (fbp) of Listeria monocytogenes (P. Gilot, Y. Jossin, and J. Content, J. Med. Microbiol., 49:887-896, 2000). This L. welshimeri DNA fragment expresses a 24.8-kDa protein that binds to human fibronectin. Based on the fbp sequences, we developed novel PCR assays for the identification of L. welshimeri and L. monocytogenes.

* Corresponding author. Present address: Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, Domaine de l'Orfrasière, 37380 Nouzilly, France. Phone: 33-2-47 42 78 78. Fax: 33-2-47 42 77 79. E-mail: gilot{at}tours.inra.fr.

Journal of Clinical Microbiology, February 2002, p. 698-703, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.698-703.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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