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Journal of Clinical Microbiology, February 2002, p. 707-711, Vol. 40, No. 2
0095-1137/01/$04.00+0     DOI: 10.1128/JCM.40.2.707-711.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense Strains Based on 16S-23S Ribosomal DNA Internal Transcribed Spacer Sequences

Department of Preventive Medicine and Public Health, Faculty of Veterinary Science, University of Khartoum, Khartoum North, Sudan,¹ Institut für Mikrobiologie und Immunologie, Lungenklinik Heckeshorn, 14109 Berlin,² TIB Molbiol, 10829 Berlin,³ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, 38124 Braunschweig, Germany,⁴ Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom⁵

Received 21 May 2001/ Returned for modification 9 October 2001/ Accepted 2 December 2001

16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes and M. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the M. fortuitum complex. M. farcinogenes and M. senegalense were indistinguishable in their 5"-end 16S rDNA but showed both considerable interspecies spacer sequence divergence and a high level of intraspecies sequence stability. A rapid detection assay using PCR and hybridization with species-specific probes was developed. The assay was specific among 46 species other than M. farcinogenes and M. senegalense and correctly identified all M. farcinogenes and M. senegalense strains. PCR- and 16S-23S rDNA sequence-based detection will be a valuable approach for diagnosis of the causal agents of African bovine farcy in cattle.

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