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Journal of Clinical Microbiology, March 2002, p. 1060-1062, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1060-1062.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal

Carmen Aldea, Carmen P. Alvarez, Lola Folgueira, Rafael Delgado, and Joaquín R. Otero*

Servicio de Microbiología, Hospital 12 de Octubre, Madrid, Spain

Received 8 November 2001/ Accepted 4 December 2001

We have evaluated a real-time PCR procedure based on the LightCycler technology for rapid detection of herpes simplex virus (HSV) in genital lesions. Two sets of primers, corresponding to the thymidine kinase and DNA polymerase regions, were used for the amplification reactions in separate capillaries containing the SYBR Green I dye as detection signal. In 28 of 118 samples (24%), HSV was isolated by conventional cell culture. All cell culture-positive samples were also positive by real-time PCR. Six additional cell culture-negative samples were positive by PCR with both sets of primers. Total processing time was less than 3 h. Real-time PCR using SYBR Green I as detection signal is a sensitive procedure for the rapid diagnosis of HSV in genital lesions.

* Corresponding author. Mailing address: Unidad de Virología, Servicio de Microbiología, Hospital 12 de Octubre, Madrid 28041, Spain. Phone: 34 91 3908428. Fax: 34 91 5652765. E-mail: jrotero{at}hdoc.insalud.es.

Journal of Clinical Microbiology, March 2002, p. 1060-1062, Vol. 40, No. 3
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.3.1060-1062.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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